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Coral Harvest Yields Fluorescent Light Switch
Reversible tag eases protein monitoring studies
The Scientist 2005, 19(3):33
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A group of researchers at RIKEN in Japan have developed a fluorescent protein that can be switched on and off more than 100 times.[1]
Atsushi Miyawaki and colleagues engineered monomeric fluorescent protein 22Gm3 (renamed "Dronpa") from the coral Pectiniidae and found that strong irradiation at 488 nm converts it to a nonfluorescent state absorbing at 390 nm. This darkened protein can be switched back to its original emission state by irradiation for several minutes at 405 nm.
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