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Tag! Purifying Proteins with Affinity Chromatography
Polyhistidine and glutathione-S-transferase lead the pack in affinity chromatography resins
Email: Aileen Constans - aconstans@the-scientist.com The Scientist 2005, 19(4):30
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What is now a standard protein laboratory technique began as an act of desperation. In the late 1960s, two scientists in Christian Anfinsen's lab at the National Institute of Arthritis and Metabolic Diseases sought a way to purify large quantities of staphylococcal nuclease for enzyme-inhibitor interaction studies. At the time, the only chromatographic methods for purifying proteins took advantage of the physical properties of hydrophobicity, size, and charge, and single purification procedures required multiple steps.
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