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Eek, No Mouse!
New hESC culture method eliminates mouse feeder cells
The Scientist 2005, 19(8):33
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To encourage human embryonic stem cells (hESCs) to multiply without differentiating, scientists currently use a complex chemical mixture provided by mouse embryonic fibroblast "feeder cells." But these nonhuman cells pose problems for therapeutic hESC use. Now a group of researchers has shown how to avoid the mouse products by incorporating basic fibroblast growth factor (bFGF) and noggin, an inhibitor of bone morphogenic protein (BMP), into the growth medium.
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