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New Amines on the Block
Synthetic amino acids aid understanding of large protein complexes
The Scientist 2005, 19(11):34
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Scientists searching for protein-protein interactions generally must look for them in vitro. But available techniques, such as chemical crosslinking and coimmunoprecipitation, are prone to false-positive and false-negative results. Cell lysis procedures, for instance, may bring into contact proteins that normally are compartmentalized in the cell, while wash procedures can dissociate fragile intermolecular interactions. And uncontrolled chemical methods can make even the most solitary polypeptide seem promiscuous.
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