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Whole-Genome Genotyping on Microarrays
New array-based SNP genotyping assay eliminates need for PCR
The Scientist 2005, 19(13):36
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To map most of the genetic variation in the human genome, scientists need to look at several hundred thousand single nucleotide polymorphisms (SNPs) per individual. Most genotyping assays employ polymerase chain reaction (PCR) to make the genome's 3 billion base complexity manageable. But ideally these assays would mirror gene expression and employ a single-tube assay to readout all the loci in an array-based assay.
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