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Don't Release This Catch!
Immunoprecipitation system overcomes problems with traditional methods
The Scientist 2005, 19(14):34
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Researchers often use immunoprecipitation (IP) to isolate specific proteins from complex samples or to identify protein-protein interactions. But the method, in which an antibody bound to protein A or G and tethered to a resin pulls an antigen out of a protein mixture, involves a number of liquid-handling steps that can lower yield, limit reproducibility, and preclude high-throughput processing. Plus, the resin (typically agarose beads) can interfere sterically with downstream assays, as it is difficult to dissociate it from the antigen.
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