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Cloning Without Restriction
With recombinase-mediated cloning systems, worry about inconvenient or absent restriction sites becomes a thing of the past
Email: Gail Dutton - gdutton@the-scientist.com The Scientist 2005, 19(17):32
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Cloning DNA fragments using restriction enzymes is like flying from Seattle to New York via Phoenix. You arrive at your destination, but it takes longer than necessary to get there. Restriction sites, like airline hubs, aren't always where you need them, and sometimes they aren't there at all.
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