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Modified primers
Email: Jonathan B Weitzman - jonathanweitzman@hotmail.com News from The Scientist 2002, 3(1):20020701-01
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A limiting aspect of microarray experiments is the efficient production of labelled cDNA probes from small amounts of starting material (typically over 20 μg of total RNA is required) . In the July issue of Nature Biotechnology, Charlie Xiang and colleagues describe a technique for efficient and inexpensive probe production using random hexamer oligonucleotide primers that have been modified with free amino groups at their 5' ends (Nat Biotechnol 2002, 20:738-742).
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