Upgrade Your Lab to TIRF

By Jeffrey M. Perkel

Why spend six figures on a new total internal reflection fluorescence system when you can upgrade your existing microscope for just $30,000?

Total internal reflection fluorescence (TIRF) microscopy is a method that resolves molecular events specifically at or near the cell surface. Follow this step-by-step guide, complete with pictures, to learn how to upgrade your existing microscope in as little as half an hour.

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Beyond GFP

By Chandra Shekar

Fluorescent repertoire expands with oranges, cherries

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Papers to Watch: Replication factories in action

By The Scientist Staff

Investigators now have experimental evidence that two replication forks diverging from a single origin are held together in space.

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Lipid Rafts' Failure to Launch

By Jill U. Adams

Debating what binds membrane microdomains

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Papers to Watch: Cell division rewinds

By The Scientist Staff

The cell cycle is generally considered a one way ticket to division. Oklahoma Medical Research Foundation scientists recently reversed the process.

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Papers to Watch: Membrane fusion model de-fused

By The Scientist Staff

Researchers have now shown that SNARE complex formation does not automatically lead to membrane fusion.

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Spotlight

The Handbook - A Guide to Fluorescent Probes and Labeling Technologies
Newly revised, The Handbook contains detailed information describing the use of more than 3000 Molecular Probes products for life science research, including extensive data, numerous technical notes, and full-color images of the products in action.

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Products for State-of-the-Art Imaging
Products for State-of-the-Art Imaging offers a concise overview of conjugates and reagents for cutting-edge imaging solutions. This eleven-page publication provides details on our full range of Alexa Fluor secondary antibody conjugates (including cross-adsorption data), and describes powerful tools for image optimization-ProLong Gold antifade reagent and Image-iT FX signal enhancer.

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Fluorescence Spectraviewer
Now you can plot and compare spectra and check the spectral compatibility for many fluorophores offered by Molecular Probes.

PubMed Articles

Real-time, multidimensional in vivo imaging used to investigate blood flow in mouse pancreatic islets.

J Clin Invest. 2008 Oct 9;
Nyman LR, Wells KS, Head WS, McCaughey M, Ford E, Brissova M, Piston DW, Powers AC

Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy.

Science. 2008 Oct 9;
Keller PJ, Schmidt AD, Wittbrodt J, Stelzer EH

Direct Visualization of Reversible Switching of Micropatterned Polyelectrolyte Brushes on Gold Surfaces Using Laser Scanning Confocal Microscopy.

Langmuir. 2008 Oct 9;
Dai X, Zhou F, Khan N, Huck WT, Kaminski CF

Surface Organization and Cooperativity during Nonspecific Protein Adsorption Events.

J Phys Chem B. 2008 Oct 9;
Rabe M, Verdes D, Zimmermann J, Seeger S

Atomic Force Microscopy Imaging and Dilute Solution Properties of Cyclic and Linear Polystyrene Combs.

J Am Chem Soc. 2008 Oct 8;
Schappacher M, Deffieux A

On the mechanism of organelle degradation in the vertebrate lens.