Upgrade Your Lab to TIRF

By Jeffrey M. Perkel

Why spend six figures on a new total internal reflection fluorescence system when you can upgrade your existing microscope for just $30,000?

Total internal reflection fluorescence (TIRF) microscopy is a method that resolves molecular events specifically at or near the cell surface. Follow this step-by-step guide, complete with pictures, to learn how to upgrade your existing microscope in as little as half an hour.

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Beyond GFP

By Chandra Shekar

Fluorescent repertoire expands with oranges, cherries

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Papers to Watch: Replication factories in action

By The Scientist Staff

Investigators now have experimental evidence that two replication forks diverging from a single origin are held together in space.

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Lipid Rafts' Failure to Launch

By Jill U. Adams

Debating what binds membrane microdomains

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Papers to Watch: Cell division rewinds

By The Scientist Staff

The cell cycle is generally considered a one way ticket to division. Oklahoma Medical Research Foundation scientists recently reversed the process.

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Papers to Watch: Membrane fusion model de-fused

By The Scientist Staff

Researchers have now shown that SNARE complex formation does not automatically lead to membrane fusion.

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Spotlight

The Handbook - A Guide to Fluorescent Probes and Labeling Technologies
Newly revised, The Handbook contains detailed information describing the use of more than 3000 Molecular Probes products for life science research, including extensive data, numerous technical notes, and full-color images of the products in action.
Spotlight

Products for State-of-the-Art Imaging
Products for State-of-the-Art Imaging offers a concise overview of conjugates and reagents for cutting-edge imaging solutions. This eleven-page publication provides details on our full range of Alexa Fluor secondary antibody conjugates (including cross-adsorption data), and describes powerful tools for image optimization-ProLong Gold antifade reagent and Image-iT FX signal enhancer.
Spotlight

Fluorescence Spectraviewer
Now you can plot and compare spectra and check the spectral compatibility for many fluorophores offered by Molecular Probes.
PubMed Articles
Synthesis of PVP-coated ultra-small Fe(3)O (4) nanoparticles as a MRI contrast agent.
J Mater Sci Mater Med. 2010 Feb 7;
Zhang Y, Liu JY, Ma S, Zhang YJ, Zhao X, Zhang XD, Zhang ZD
From Dynamic Live Cell Imaging to 3D Ultrastructure: Novel Integrated Methods for High Pressure Freezing and Correlative Light-Electron Microscopy.
PLoS One. 2010; 5(2): e9014
Spiegelhalter C, Tosch V, Hentsch D, Koch M, Kessler P, Schwab Y, Laporte J
Nanobubble-assisted formation of carbon nanostructures on basal plane highly ordered pyrolytic graphite exposed to aqueous media.
Nanotechnology. 2010 Mar 5; 21(9): 95707
Janda P, Frank O, Bastl Z, Klementová M, Tarábková H, Kavan L
Magnetic Resonance Molecular Imaging of Thrombosis in an Arachidonic Acid Mouse Model Using an Activated Platelet Targeted Probe.
Arterioscler Thromb Vasc Biol. 2010 Feb 5;
Klink A, Lancelot E, Ballet S, Vucic E, Fabre JE, Gonzalez W, Medina C, Corot C, Mulder WJ, Mallat Z, Fayad ZA
Characterization of different tissue changes in normal, betel chewers, potentially malignant lesions, conditions and oral squamous cell carcinoma using reflectance confocal microscopy: Correlation with routine histopathology.
Oral Oncol. 2010 Feb 5;
Anuthama K, Sherlin HJ, Anuja N, Ramani P, Premkumar P, Chandrasekar T
Morphological/chemical imaging of demineralized dentin layer in its natural, wet state.
Dent Mater. 2010 Feb 5;
Wang Y, Yao X
Visualization of feline calicivirus replication in real-time with recombinant viruses engineered to express fluorescent reporter proteins.
Virology. 2010 Feb 3;
Abente EJ, Sosnovtsev SV, Bok K, Green KY
Thinned-skull cranial window technique for long-term imaging of the cortex in live mice.
Nat Protoc. 2010 Jan; 5(2): 201-8
Yang G, Pan F, Parkhurst CN, Grutzendler J, Gan WB
Formation and dissociation of M1 muscarinic receptor dimers seen by total internal reflection fluorescence imaging of single molecules.
Proc Natl Acad Sci U S A. 2010 Jan 20;
Hern JA, Baig AH, Mashanov GI, Birdsall B, Corrie JE, Lazareno S, Molloy JE, Birdsall NJ
Layer-specific variation of iron content in cerebral cortex as a source of MRI contrast.
Proc Natl Acad Sci U S A. 2010 Feb 3;
Fukunaga M, Li TQ, van Gelderen P, de Zwart JA, Shmueli K, Yao B, Lee J, Maric D, Aronova MA, Zhang G, Leapman RD, Schenck JF, Merkle H, Duyn JH
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