Upgrade Your Lab to TIRF

By Jeffrey M. Perkel

Why spend six figures on a new total internal reflection fluorescence system when you can upgrade your existing microscope for just $30,000?

Total internal reflection fluorescence (TIRF) microscopy is a method that resolves molecular events specifically at or near the cell surface. Follow this step-by-step guide, complete with pictures, to learn how to upgrade your existing microscope in as little as half an hour.

Full Text

Beyond GFP

By Chandra Shekar

Fluorescent repertoire expands with oranges, cherries

Full Text

Papers to Watch: Replication factories in action

By The Scientist Staff

Investigators now have experimental evidence that two replication forks diverging from a single origin are held together in space.

Full Text

Lipid Rafts' Failure to Launch

By Jill U. Adams

Debating what binds membrane microdomains

Full Text

Papers to Watch: Cell division rewinds

By The Scientist Staff

The cell cycle is generally considered a one way ticket to division. Oklahoma Medical Research Foundation scientists recently reversed the process.

Full Text

Papers to Watch: Membrane fusion model de-fused

By The Scientist Staff

Researchers have now shown that SNARE complex formation does not automatically lead to membrane fusion.

Full Text

Spotlight

The Handbook - A Guide to Fluorescent Probes and Labeling Technologies
Newly revised, The Handbook contains detailed information describing the use of more than 3000 Molecular Probes products for life science research, including extensive data, numerous technical notes, and full-color images of the products in action.
Spotlight

Products for State-of-the-Art Imaging
Products for State-of-the-Art Imaging offers a concise overview of conjugates and reagents for cutting-edge imaging solutions. This eleven-page publication provides details on our full range of Alexa Fluor secondary antibody conjugates (including cross-adsorption data), and describes powerful tools for image optimization-ProLong Gold antifade reagent and Image-iT FX signal enhancer.
Spotlight

Fluorescence Spectraviewer
Now you can plot and compare spectra and check the spectral compatibility for many fluorophores offered by Molecular Probes.
PubMed Articles
Wide-field optical nanoprofilometry using structured illumination.
Opt Lett. 2009 Nov 15; 34(22): 3538-3540
Wang CC, Lee KL, Lee CH
Improved spatial resolution in fluorescence focal modulation microscopy.
Opt Lett. 2009 Nov 15; 34(22): 3508-3510
Gong W, Si K, Chen N, Sheppard CJ
Probing cellular mechanobiology in three-dimensional culture with collagen-agarose matrices.
Biomaterials. 2009 Nov 17;
Ulrich TA, Jain A, Tanner K, Mackay JL, Kumar S
Immunofluorescence microscopy for imaging of nuclear p63 in human primary keratinocytes: A comparison of antibodies and fixation methods.
J Immunol Methods. 2009 Nov 16;
Markert CD, Bharadwaj S, Zhang Y, Childers MK, Furth ME
AFM Visualization of Clathrin Triskelia under Fluid and in Air.
FEBS Lett. 2009 Nov 16;
Kotova S, Prasad K, Smith PD, Lafer EM, Nossal R, Jin AJ
Quantifying light scattering with single-mode fiber-optic confocal microscopy.
BMC Med Imaging. 2009 Nov 19; 9(1): 19
Lacroix JT, Haidekker MA
Visualization of melatonin's multiple mitochondrial levels of protection against mitochondrial Ca-mediated permeability transition and beyond in rat brain astrocytes.
J Pineal Res. 2009 Nov 17;
Jou MJ, Peng TI, Hsu LF, Jou SB, Reiter RJ, Yang CM, Chiao CC, Lin YF, Chen CC
Melamine Acoustic Chemosensor Based on Molecularly Imprinted Polymer Film.
Anal Chem. 2009 Nov 19;
Pietrzyk A, Kutner W, Chitta R, Zandler ME, D'Souza F, Sannicolo? F, Mussini PR
Multifunctional Block Copolymer Micelles for the Delivery of (111)In to EGFR-Positive Breast Cancer Cells for Targeted Auger Electron Radiotherapy.
Mol Pharm. 2009 Nov 19;
Fonge H, Lee H, Reilly RM, Allen C
Emission enhancement within gold spherical nanocavity arrays.
Phys Chem Chem Phys. 2009 Dec 14; 11(46): 10923-10933
Jose B, Steffen R, Neugebauer U, Sheridan E, Marthi R, Forster RJ, Keyes TE
Email: Password:
Forgot Password?
New Users?
This month:

Front Cover

Today:

The Daily:
Sign up for The Scientist's daily e-mail.

Blogs:
Latest Posts

This week:

Podcast:
TheWeek

Now on iTunes
subscribe now