Upgrade Your Lab to TIRF

By Jeffrey M. Perkel

Why spend six figures on a new total internal reflection fluorescence system when you can upgrade your existing microscope for just $30,000?

Total internal reflection fluorescence (TIRF) microscopy is a method that resolves molecular events specifically at or near the cell surface. Follow this step-by-step guide, complete with pictures, to learn how to upgrade your existing microscope in as little as half an hour.

Full Text

Beyond GFP

By Chandra Shekar

Fluorescent repertoire expands with oranges, cherries

Full Text

Papers to Watch: Replication factories in action

By The Scientist Staff

Investigators now have experimental evidence that two replication forks diverging from a single origin are held together in space.

Full Text

Lipid Rafts' Failure to Launch

By Jill U. Adams

Debating what binds membrane microdomains

Full Text

Papers to Watch: Cell division rewinds

By The Scientist Staff

The cell cycle is generally considered a one way ticket to division. Oklahoma Medical Research Foundation scientists recently reversed the process.

Full Text

Papers to Watch: Membrane fusion model de-fused

By The Scientist Staff

Researchers have now shown that SNARE complex formation does not automatically lead to membrane fusion.

Full Text

Spotlight

The Handbook - A Guide to Fluorescent Probes and Labeling Technologies
Newly revised, The Handbook contains detailed information describing the use of more than 3000 Molecular Probes products for life science research, including extensive data, numerous technical notes, and full-color images of the products in action.
Spotlight

Products for State-of-the-Art Imaging
Products for State-of-the-Art Imaging offers a concise overview of conjugates and reagents for cutting-edge imaging solutions. This eleven-page publication provides details on our full range of Alexa Fluor secondary antibody conjugates (including cross-adsorption data), and describes powerful tools for image optimization-ProLong Gold antifade reagent and Image-iT FX signal enhancer.
Spotlight

Fluorescence Spectraviewer
Now you can plot and compare spectra and check the spectral compatibility for many fluorophores offered by Molecular Probes.
PubMed Articles
LED arrays as cost effective and efficient light sources for widefield microscopy.
PLoS ONE. 2008; 3(5): e2146
Albeanu DF, Soucy E, Sato TF, Meister M, Murthy VN
Epifluorescence Imaging of Electrochemically Switchable Langmuir-Blodgett Films of Nafion.
Langmuir. 2008 May 13;
Moretto LM, Kohls T, Chovin A, Sojic N, Ugo P
Tektin 2 is required for central spindle microtubule organization and the completion of cytokinesis.
J Cell Biol. 2008 May 12;
Durcan TM, Halpin ES, Rao T, Collins NS, Tribble EK, Hornick JE, Hinchcliffe EH
Ambient Molecular Imaging and Depth Profiling of Live Tissue by Infrared Laser Ablation Electrospray Ionization Mass Spectrometry.
Anal Chem. 2008 May 13;
Nemes P, Barton AA, Li Y, Vertes A
Ultrashort electron pulses for diffraction, crystallography and microscopy: theoretical and experimental resolutions.
Phys Chem Chem Phys. 2008 May 28; 10(20): 2894-909
Gahlmann A, Tae Park S, Zewail AH
4D electron imaging: principles and perspectives.
Phys Chem Chem Phys. 2008 May 28; 10(20): 2879-93
Shorokhov D, Zewail AH
In vivo confocal fluorescence imaging of the intratumor distribution of the photosensitizer mono-L-aspartylchlorin-e6.
Neoplasia. 2008 May; 10(5): 429-38
Mitra S, Foster TH
Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples.
Nat Methods. 2008 May 11;
Juette MF, Gould TJ, Lessard MD, Mlodzianoski MJ, Nagpure BS, Bennett BT, Hess ST, Bewersdorf J
Detection and imaging of non-contractile inclusions and sarcomeric anomalies in skeletal muscle by second harmonic generation combined with two-photon excited fluorescence.
J Struct Biol. 2008 Apr 8;
Ralston E, Swaim B, Czapiga M, Hwu WL, Chien YH, Pittis MG, Bembi B, Schwartz O, Plotz P, Raben N
Diameter-selective solubilization of carbon nanotubes by lipid micelles.
J Nanosci Nanotechnol. 2008 Jan; 8(1): 420-3
Tasis D, Papagelis K, Douroumis D, Smith JR, Bouropoulos N, Fatouros DG
Email: Password:
Forgot Password?
New Users?
This month:

Front Cover

Today:

The Daily:
Sign up for The Scientist's daily e-mail.

Blogs:
Latest Posts

This week:

Podcast:
TheWeek

Now on iTunes
subscribe now