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Diffusion in the Ranks
Michael Brush | Feb 4, 2001 | 8 min read
Suppliers of Dialysis Tubing Suppliers of Dialysis Devices (Not included in print edition) Dialysis effects the simple, gentle, and gradual separation of biological and other molecules from unwanted molecules in solution by selective diffusion through a semipermeable membrane. In practice, a sample of protein or nucleic acid that contains an unwanted small molecular weight compound, such as a buffer salt, is placed on one side of a semipermeable membrane. The dialysate, a solution of low ionic
Plantsā€™ Epigenetic Secrets
Jef Akst | Feb 1, 2017 | 10+ min read
Unlike animals, plants stably pass on their DNA methylomes from one generation to the next. The resulting gene silencing likely hides an abundance of phenotypic variation.
Frontlines
Hal Cohen | Feb 17, 2002 | 5 min read
New evidence points to brain trauma as an environmental risk factor for Alzheimer disease (AD). 
Evolution of Sexual Dimorphism
Ricki Lewis | Feb 4, 2001 | 4 min read
Sexual dimorphism makes life interesting for many species. In the case of the fruit fly Drosophila melanogaster, the rear end of a male is so much darker than that of a female that a seasoned fly pusher can distinguish he from she even without the aid of a microscope. A telling investigation by Artyom Kopp and Sean Carroll at the Howard Hughes Medical Institute and Laboratory of Molecular Biology at the University of Wisconsin-Madison and Ian Duncan at the Department of Biology at Washington Uni
New Products
The Scientist Staff | Feb 2, 1997 | 9 min read
The ABI PRISM 877 Integrated Thermal Cycler complements Perkin-Elmer Applied Biosystems' offerings for DNA sequencing and analysis, which include instrumentation, reagents, and data-management products. The 384-well thermal cycler is integrated with a robot capable of pipetting extremely low volumes necessary for both polymerase chain reaction (PCR) and sequencing reactions, enabling these operations to be carried out on a single platform. Users may choose to run preprogrammed protocols or c
Developing Intellectual Entrepreneurship
Kate Devine | Mar 4, 2001 | 4 min read
Doctoral programs do not adequately prepare students for the future. So says the recent study funded by the Pew Charitable Trusts, "At Cross Purposes: What the Experiences of Today's Doctoral Students Reveal about Doctoral Education."1 To solve this problem the University of Texas, which produces the largest number of Ph.D.s annually, established a professional development program (www.utexas.edu/ogs/development.html). Initiated in 1997, the mission of the University of Texas at Austin Intellect
Making Sense of Microchip Array Data
Michael Brush | Apr 29, 2001 | 9 min read
Microarray Analysis Software Packages     Courtesy of SpotfireSpotfire's Array Explorer Gene expression profiling using DNA microarrays generates reams of data. But as is so often the case, it's not the quantity but the quality that matters: Gene expression data is useless unless biologically meaningful information can be extracted and presented in some readily understandable fashion. The production of this meaningful information, involving many facets of image processing, statistic
Keeping Time with Drosophila
Laura Bonetta | Feb 3, 2002 | 10 min read
Circadian clocks—the biological timekeepers that operate on a daily cycle—keep virtually every living creature in tune with its environment. These internal clocks regulate a wide range of fundamental biological processes, including movement, smell, sleep, mating, and feeding. A true circadian clock is endogenous; that is, it keeps time even in the absence of external cues. The clock can, however, be reset, or entrained, by daylight, allowing the synchronization of circadian rhythms t
Frontlines
Hal Cohen | Feb 3, 2002 | 5 min read
The proper diet for longevity may not be what is eaten, but what is not. University of California, Los Angeles, researchers have reported that the withdrawal of coenzyme Q (Q) from the diet of Caenorhabditis elegans extends the adult life span by almost 60% (P.L. Larsen, C.F. Clarke, "Extension of life-span in Caenorhabditis elegans by a diet lacking coenzyme Q," Science, 295:120-3, Jan. 4, 2002.) Q is found in the respiratory chains of mitochondria and can be obtained from eating anything that
Mutants Made Easy
Jeffrey Perkel | Feb 4, 2001 | 8 min read
Suppliers of in vitro Site-directed Mutagenesis Kits Biological research greatly benefits from the ability to introduce specific mutations into a DNA sequence. Researchers use site-directed mutagenesis procedures to precisely analyze individual amino acid residues in a protein sequence and in specific protein-nucleic acid interactions. Likewise, serial deletion and random insertion protocols can ease protein structural studies and promoter analyses. In their original incarnations, site-directe

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