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tag cleavage plane genetics genomics immunology

Top 10 Innovations 2013
The Scientist | Dec 1, 2013 | 10+ min read
The Scientist’s annual competition uncovered a bonanza of interesting technologies that made their way onto the market and into labs this year.
Bioterrorism Research: New Money, New Anxieties
John Dudley Miller | Apr 6, 2003 | 8 min read
Ned Shaw US scientists have reason to feel both heady and scared. The federal government recently released unprecedented billions of dollars to fund bioterrorism research. Yet, the merits of this sudden shift in focus are being debated, and some worry that the money will be squandered or wasted. "I have been really very upset by the focus on bioterrorism," says Stanley Falkow, professor of microbiology and immunology and of medicine at Stanford University. "Everybody's talking about it, but th
Notable
Jeffrey Perkel | Jan 6, 2002 | 4 min read
J.A. Camarero et al., "Peptide chemical ligation inside living cells: In vivo generation of a circular protein domain," Bioorganic and Medicinal Chemistry, 9[9]:2479-84, September 2001. F1000 Rating: Recommended "To investigate whether chemo-selective peptide ligations work in vivo, the authors demonstrate the in vivo head-to-tail cyclization of a bioactive SH3 domain using native chemical ligation. The protein precursor was constructed using a clever combination of an intein-fusion protein an
Recourse to Death
Michael Brush | Aug 20, 2000 | 10 min read
Manufactureres of Flow Cytometry Products for Apoptosis Detection To examine the causes of life, we must first have recourse to death," uttered Victor Frankenstein upon beginning his search for the source from "whence the principle of life proceeds" and ultimately creating his nameless monster. Frankenstein's real creator, 19-year-old Mary Shelley, probably had no idea when Frankenstein was first published in 1816 that her main character's motivation would eventually have real-life implication
A Look at Drosophila Pattern Formation
Jeffrey Perkel | Sep 2, 2001 | 9 min read
Researchers interested in gene expression studies adopt one of two approaches. They can either examine the expression of a given gene in a population of cells in aggregate, or they can study the gene on a cell-by-cell basis in situ. The advantage of the former approach is its simplicity: It is generally easy to prepare RNA or protein from a given tissue sample and to probe it for the gene or protein of interest. But there are several disadvantages associated with the population approach. First o

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