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tag quantitative pcr cell molecular biology developmental biology neuroscience genetics genomics

Green and red fluorescent proteins in a zebrafish outline the animal’s vasculature in red and lymphatic system in green in a fluorescent image. Where the two overlap along the bottom of the animal is yellow.
Serendipity, Happenstance, and Luck: The Making of a Molecular Tool
Shelby Bradford, PhD | Dec 4, 2023 | 10+ min read
The common fluorescent marker GFP traveled a long road to take its popular place in molecular biology today.
A scientist loads a 96-well plate containing qPCR reactions into a thermal cycler for DNA amplification and detection.
Insights into qPCR: Protocol, Detection Methods, and Analysis
Tanuka Biswas, PhD | Nov 8, 2023 | 6 min read
Learn about quantitative PCR (qPCR), including its fundamentals, common applications, modes of detection, and key quantification methods.
Molecule of DNA forming inside the test tube equipment.3d rendering,conceptual image.
EvaGreen® Dye: The Swiss Army Knife of qPCR
Biotium | Mar 1, 2024 | 7 min read
A green fluorescent dye with a novel DNA binding mechanism improves signal-to-noise in different DNA amplification assays.
TSS
Smart Gateways into the Lab of the Future
The Scientist | Sep 21, 2023 | 2 min read
Neurobiologists, computer scientists, and engineers join forces to grow mini brains using automation and make their technologies smarter with artificial intelligence.
PCR tubes placed into the 96-well loading chamber of a PCR thermocycler instrument.
Directing Superior Reagents for Better PCR Results
The Scientist and MilliporeSigma | Oct 2, 2023 | 3 min read
Directed evolution approaches are creating new reagents to help a tried-and-true technique reach new heights.
Optimizing PCR: Proven Tips and Troubleshooting Tricks
Optimizing PCR: Proven Tips and Troubleshooting Tricks
Tanuka Biswas, PhD | Feb 23, 2024 | 10 min read
Scientists optimize PCR to ensure successful amplification and analysis.
Quantitative PCR Update
Wendy Gloffke | Apr 20, 2003 | 9 min read
Courtesy of Applied Biosystems  THE TAQMAN PROCEDURE: Applied Biosystems' TaqMan procedure relies on the 5'-to-3' exonuclease activity of Taq polymerase. The TaqMan probe bears a fluoro-phore at the 5' end and a quencher on the 3' end, rendering the molecule non-fluorescent. During amplification, the probe binds to the template between the two PCR primers. When the polymerase encounters the probe, it starts chewing away at the end, releasing the fluorophore into solution, where it is free
Bio-Rad 
Leveraging Molecular Technologies to Stay on Top of the Scientific Game
The Scientist Staff | Oct 26, 2022 | 5 min read
Joel Pearson discusses how emerging genomic technologies are shaping his cancer research.
On the left is a normally developing mouse embryo, on the right is a slightly larger mouse embryo that also contains horse cells that glow green.
Chimera research opens new doors to understanding and treating disease
Hannah Thomasy, PhD, Drug Discovery News | Aug 9, 2023 | 10 min read
Animals with human cells could provide donor organs or help us understand neuropsychiatric disorders.
Exploring the Past, Present, and Future of Brain Organoids 
Niki Spahich, PhD | Feb 18, 2024 | 5 min read
Paola Arlotta seeks to understand the complex symphony of brain development in vitro by using organoid models.

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