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tag transfection culture

Protein Expression Without Transfection
Elizabeth Mckenna | Jul 21, 2002 | 2 min read
Protein transfection, also called transduction, is the delivery of a biologically active protein or peptide directly into living cells.1 Pro-Ject™, a cationic lipid-based reagent, from Rockford, Ill.-based Pierce Biotechnology accomplishes this noninvasively--first, by noncovalently complexing with a protein, then fusing with or being endocytosed by the cell membrane, and finally, delivering a functional protein into the cytoplasm. Pro-Ject is fast and simple to use, with an average kit
Ready-to-Use Tagged cDNA Clones
AMSbio | Jun 1, 2015 | 1 min read
Available from AMSBIO TrueORF®cDNA clones are tagged cDNA clones for protein studies.
No Barriers to Entry: Transfection tools get biomolecules in the door
Mitra Ostresh | May 23, 1999 | 8 min read
Date: May 24, 1999Relative Transfection Efficiency Transfection Reagents Table and Electroporation Systems Table Model of the Effectene principle. The enhancer first condenses the plasmid DNA and the effectene reagent, non- liposomal lipid, subsequently coats the condensed DNA for efficient uptake into cells. Supplied by QIAGEN. Cell transformation is an essential tool for molecular, cellular, and genetic research. Introducing specific molecules--DNA, RNA, drugs, and small molecules such as ca
New Products
The Scientist Staff | Feb 5, 1995 | 3 min read
The Ni-NTA spin columns provide purification of 6xHis-tagged proteins from small-scale expression cultures. Available in two formats--separately without reagents or as a complete kit--they can be used in experimental situations requiring small-scale expression, functional screening, and rapid purification of engineered and recombinant proteins. Each can purify up to 100 fg of 6xHis tagged protein under native or denaturing conditions. According to the manufacturer, this procedure allows 12 mini
Luminescence Developments Help Scientists See The Light
James Kling | May 11, 1997 | 9 min read
Biologists are constantly seeking more sensitive assays to detect the presence of organisms or telltale DNA, RNA, and proteins. Although radioactive tags incorporated into the target itself (or into a complementary strand)-and later detected by Geiger counters or film exposures-have traditionally given good sensitivity, the problems of waste disposal and laboratory monitoring have driven a search for alternative tags that have radioactivity's sensitivity but avoid its hazards. Fluorescent tags-
Making Things Grow: Insect Cells, Stem Cells, and Primary Cell Lines All Pose Challenges for Cell Culturists
Laura Defrancesco | Jun 21, 1998 | 5 min read
Date: June 22, 1998 Insect Cell Culture Media, Suppliers of Primary Cell Culture Media Advantages for Protein Expression Studies Since the mid-1950s cultures of insects--cockroaches, fruit flies, and leafhoppers, to name a few--have been the object of quiet study by physiologists and cell biologists. But along came genetic engineering and suddenly insect cultures have been put in the spotlight since they provide advantages over both bacterial and mammalian systems for recombinant protein prod
Bench Buys
Aileen Constans | Jul 7, 2002 | 1 min read
Image: Courtesy of Brinkmann Bacterial Economy Westbury, NY-based Brinkmann introduces the Eppendorf® LidBac, a membrane-lid tube that enables cultivation of bacterial cultures at the miniprep scale. An economical, fast alternative to conventional large-scale bacterial cultivation, the LidBac consists of a standard microcentrifuge tube with a polypropylene membrane lid and an integrated hydrophobic membrane. The culture itself grows in the tube; it can then be used for downstream applic
Buyer's Guide to Protein Transduction Reagents
Josh Roberts | Jun 6, 2004 | 5 min read
Six years ago, when John Tinsley's postdoctoral advisor at Texas A&Mtold him to find a protocol to introduce proteins into coronary endothelial cells, he couldn't find one in the literature. Tinsley tested a variety of commercial DNA transfection reagents, found one that worked for proteins, and has been using it since then.Tinsley's experience would be a lot different today. No longer must researchers rely on tedious and toxic procedures, or on proprietary reagents designed specifically for
2020 Top 10 Innovations
The Scientist | Dec 1, 2020 | 10+ min read
From a rapid molecular test for COVID-19 to tools that can characterize the antibodies produced in the plasma of patients recovering from the disease, this year’s winners reflect the research community’s shared focus in a challenging year.
New Reagents for Organoid Growth and Harvesting
AMSbio | May 5, 2016 | 2 min read
AMSBIO has introduced two new products...

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