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tag human embryonic stem cells microbiology culture ecology

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Synthetic Circuits Reveal the Key to Rewinding the Cellular Clock
Charlene Lancaster, PhD | Mar 12, 2024 | 4 min read
Using a circuit-based system, scientists determined the ideal transcription factor levels to promote the successful reprogramming of fibroblasts into induced pluripotent stem cells.
bacteria and DNA molecules on a purple background.
Engineering the Microbiome: CRISPR Leads the Way
Mariella Bodemeier Loayza Careaga, PhD | Mar 15, 2024 | 10+ min read
Scientists have genetically modified isolated microbes for decades. Now, using CRISPR, they intend to target entire microbiomes.
Julie Trolle and Jef Boeke
Self Sufficient Cells?
Katherine Irving | Jun 1, 2023 | 4 min read
Animal cells cannot produce 9 of the 20 amino acids they need to function. Some researchers are looking to change that.
Human Pluripotent Cells Pass Safety Test
Douglas Steinberg | Sep 1, 2002 | 7 min read
Image: ©2001 MacMillan Publishers Ltd.  PLURIPOTENT-CELL PIPELINE: To obtain embryonic germ cells, researchers culture primordial germ cells (PGCs) dissected from the genital ridge. If left in situ, a PGC develops into spermatozoa or ova after its imprints have been erased and reestablished. Embryonic stem cells come from the blastocyst's inner cell mass. (Reprinted with permission, Nature 414:122-8, 2001) Because the science is hard and the politics mean, progress in understanding
Stem Cells: Safe Haven For TB
Nsikan Akpan | Feb 5, 2013 | 3 min read
Tuberculosis bacteria find shelter from drugs and the body’s defenses in bone marrow stem cells.
Stem Cell Know-How
Aileen Constans | Sep 1, 2002 | 7 min read
Image: Courtesy of Gwenn-AEL Dnaet ©2002 National Academy of Sciences STEM CELL XENOGRAFT: Identification of human hepatocytes in livers from immune-deficient mice transplanted with human adult hematopoietic stem cells. Photomicrographs of NOD/SCID mouse liver sections from mice transplanted with purified human Lin-CD38-CD34-C1qRp+ cells isolated from umbilical cord blood, harvested 8-10 weeks post-transplant. Tissue sections were stained for HSA (hepatocyte-specific antigen) or c-met
a human neuron illuminated in bright green on a black background.
Mitochondrial Metabolism Dictates Neurons’ Growth Rate
Katherine Irving | Jan 30, 2023 | 4 min read
Altering the rate of respiration in mitochondria changes how fast neurons grow, making mouse neurons grow more like human ones and vice versa, a study finds.
Stem Cell Discoveries Stir Debate
Douglas Steinberg | Nov 12, 2000 | 9 min read
Editor's Note: This is the first of two articles on questions raised by recent stem cell discoveries. The second article, focusing on various organs and the nervous system, will appear in the Nov. 27 issue of The Scientist. Researchers first isolated embryonic stem cells (ESCs) from mouse blastocysts almost 20 years ago, and a paper announcing the discovery of human ESCs emerged in 1998. Adult-derived stem cells (ASCs) have since become the rage in certain quarters of biology, with unexpected--
Making Things Grow: Insect Cells, Stem Cells, and Primary Cell Lines All Pose Challenges for Cell Culturists
Laura Defrancesco | Jun 21, 1998 | 5 min read
Date: June 22, 1998 Insect Cell Culture Media, Suppliers of Primary Cell Culture Media Advantages for Protein Expression Studies Since the mid-1950s cultures of insects--cockroaches, fruit flies, and leafhoppers, to name a few--have been the object of quiet study by physiologists and cell biologists. But along came genetic engineering and suddenly insect cultures have been put in the spotlight since they provide advantages over both bacterial and mammalian systems for recombinant protein prod
Trials of the Heart
Christopher Thomas Scott(cscott@the-scientist.com) | Jul 3, 2005 | 5 min read
Adult human stem cells may offer the opportunity to use one of biomedical science's most promising technologies without the ethical dilemmas of embryonic cells.

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