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tag protein engineering developmental biology

DNA molecule.
Finding DNA Tags in AAV Stacks
Mariella Bodemeier Loayza Careaga, PhD | Mar 7, 2024 | 8 min read
Ten years ago, scientists put DNA barcodes in AAV vectors, creating an approach that simplified, expedited, and streamlined AAV screening. 
bacteria and DNA molecules on a purple background.
Engineering the Microbiome: CRISPR Leads the Way
Mariella Bodemeier Loayza Careaga, PhD | Mar 15, 2024 | 10+ min read
Scientists have genetically modified isolated microbes for decades. Now, using CRISPR, they intend to target entire microbiomes.
Microfluidics: Biology’s Liquid Revolution
Laura Tran, PhD | Feb 26, 2024 | 8 min read
Microfluidic systems redefined biology by providing platforms that handle small fluid volumes, catalyzing advancements in cellular and molecular studies.
Top 7 in developmental biology
Bob Grant | Oct 4, 2010 | 3 min read
Check out the hottest papers in developmental biology and related fields, as ranked by F1000
A person moving the hands of a vintage clock backwards.
Synthetic Circuits Reveal the Key to Rewinding the Cellular Clock
Charlene Lancaster, PhD | Mar 12, 2024 | 4 min read
Using a circuit-based system, scientists determined the ideal transcription factor levels to promote the successful reprogramming of fibroblasts into induced pluripotent stem cells.
Lock and key illustration
Novel Yeast-Assembly Technique Yields Living Materials
Natalia Mesa, PhD | Nov 23, 2022 | 3 min read
Researchers say structures made of the cells could potentially be used to clean up uranium from oceans, heal wounds, and more.
Protein Purification II: Affinity Tags
Aileen Constans | Feb 17, 2002 | 9 min read
Scientists working with recombinant proteins expressed in Escherichia coli probably use at least one liquid chromatography technique to purify their protein of interest. But liquid chromatography frequently requires a considerable amount of optimization, and usually involves several different chromatographic steps to rid the sample of contaminants.1 The ideal solution would be to create a resin that is completely specific to the target protein, enabling one-step purification. Affinity chromatogr
Green and red fluorescent proteins in a zebrafish outline the animal’s vasculature in red and lymphatic system in green in a fluorescent image. Where the two overlap along the bottom of the animal is yellow.
Serendipity, Happenstance, and Luck: The Making of a Molecular Tool
Shelby Bradford, PhD | Dec 4, 2023 | 10+ min read
The common fluorescent marker GFP traveled a long road to take its popular place in molecular biology today.
Abstract graphene structures
Synthetic Organelles Let Researchers Control Cell Behavior
Catherine Offord | Nov 1, 2021 | 3 min read
A technique that reversibly bundles tagged cargo into artificial membraneless compartments gives scientists the ability to switch cell processes on and off.
Optimizing PCR: Proven Tips and Troubleshooting Tricks
Optimizing PCR: Proven Tips and Troubleshooting Tricks
Tanuka Biswas, PhD | Feb 23, 2024 | 10 min read
Scientists optimize PCR to ensure successful amplification and analysis.

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