genetics, genetics & genomics, CRISPR/Cas

Machine learning algorithms predict the repairs made to DNA after Cas9 cuts.

CRISPR reagents injected into the amniotic fluid inactivated a gene in the fetuses that would normally cause lung disease and kill the mice a few hours after birth.

Researchers edited macaque embryos to induce symptoms of sleep disorders and chose one animal to clone. A bioethicist questions the study’s appropriateness.

He Jiankui says he has successfully used CRISPR-Cas9 to tweak the genes of twins born earlier this month. Some independent experts are dubious.

The Germany-based study will test an ex vivo genome-editing therapy for the inherited blood disorder β-thalassemia.

Brief electroporation appears to make T cells more receptive to new genetic material, which could speed the development of immunotherapies.

It’s the first demonstration of the technology in mammals.

Last year, researchers claimed the gene-editing method had accuracy issues, but a new whole-genome sequencing analysis by the same team finds otherwise.

Researchers have developed a variety of techniques to detect when CRISPR misses the mark.

Researchers identify antibodies for two commonly used Cas9 proteins in human blood. Investors take notice.

The first industry-sponsored CRISPR therapy is slated to be tested in humans in 2018.

With the arrival of a new class of single-nucleotide editors, researchers can target the most common type of pathogenic SNP in humans.

In lab populations of genetically engineered mosquitoes, mutations arose that blocked the gene drive’s spread and restored female fertility.

Researchers use base-editing to swap out an erroneous nucleotide responsible for a potentially life-threatening blood disorder.

Multiple DNA breaks at either the centromere or the long arm of the mouse Y chromosome cause it to fragment and disappear.

Slight sequence differences confound target sites in precision genome-editing, a study shows.

A study of off-target effects that sparked fear among investors of genome-editing companies receives methodological criticisms.

Using CRISPR-Cpf1 gene editing, researchers have fixed mutations that cause a form of muscular dystrophy in cultured human cardiomyocytes and a mouse model.

A CRISPR-Cas9–based method probes the regulatory roles of noncoding DNA sequences.

Advice on how to deploy the latest techniques in your own lab