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tag reagents workplace pcr products

Fluorescence microscopy image of cells expressing fluorescent biosensors. Green and magenta fluorescence is observed outside of the cell nuclei.
Choosing Fluorescent Reagents for Every Live Cell Application
The Scientist and MilliporeSigma | Nov 30, 2022 | 4 min read
Scientists gain unique insights into active biological processes with specific fluorescent probes, dyes, and biosensors.
Lab 2.0
Hayley Dunning | Dec 1, 2012 | 8 min read
Apps and software for improving lab productivity
Genotyping with PCR
M. Tevfik Dorak | Jun 1, 2007 | 5 min read
How to choose the right approach
Up to Speed on PCR
Deborah Fitzgerald | Nov 26, 2000 | 7 min read
Real-time PCR Systems Cepheid's Smart Cycler System PCR--a technique so common in today's laboratories that it is easy to forget its revolutionary impact--enables the specific amplification and detection of as little as a single copy of a particular nucleotide sequence. However, PCR has the potential to be used not just for the detection of specific sequences, but also for their quantification, because of the quantitative relationship between the amount of starting target sequence and the amoun
Innovations Expand Lab Power, Uses Of PCR Technique
Ricki Lewis | Jul 25, 1993 | 8 min read
The gene amplification technique invented by genetics researcher Kary Mullis on a moonlit drive through the northern California hills a decade ago--the polymerase chain reaction (PCR)-- continues to revolutionize the life sciences. Uses in molecular biology research and in diagnostic tests are proliferating, and PCR is even bringing a new molecular approach to such fields as paleontology and epidemiology. The following companies are among those supplying PCR-related products for the resear
PCR Based Cloning Kits: Something For Everybody
Laura Defrancesco | Apr 12, 1998 | 10+ min read
Date: April 13, 1998PCR Based Cloning Kits Table The End Table (PDF Format) PCR has found applications in almost every imaginable facet of molecular biology, and for many applications, looking at a band on a gel is not enough. Sequencing, expressing, mutating--all require cloning. And as it happens, cloning strategies that work for other types of DNA fragments don't work at all well, or require inordinate effort, with PCR fragments. For example, the most commonly used cloning strategy requires
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Assay for Sickle Cell Anemia Is Repurposed to Diagnose COVID-19
G.B.S.N.P. Varma | Nov 18, 2020 | 5 min read
A CRISPR-based, paper-strip test developed by Indian researchers performs as well as real-time PCR in identifying the presence of SARS-CoV-2 and returns results within an hour.
Cloning Without Bacteria?
Deborah Fitzgerald | Feb 17, 2002 | 3 min read
Invitrogen Corp. of Carlsbad, Calif., offers TOPO® Tools for those interested in an alternative to conventional, time-consuming cloning techniques. TOPO Tools provide a relatively fast way of joining various sequence elements, such as promoters and tags, to either or both ends of a PCR-amplified product, without traditional cloning procedures such as ligations, vector manipulations, or Escherichia coli transformation. Rather, this method harnesses the dual catalytic activities of Vaccinia t
PCR Primed To Spur Chain Of Applications
Holly Ahern | Jun 25, 1995 | 10+ min read
What would you do if your research interests revolved around obtaining DNA from a bacterium preserved for millions of years in the gut of a bee stuck in amber, matching up a murderer to crime- scene blood half a century old, or cloning genes from a 1,000- year-old mummy? Most scientists would first consider PCR--the polymerase chain reaction--as a technique for approaching problems such as these. With PCR, minute quantities of nucleic acids can be amplified millions of times into sufficient qua
Creative Emulsification
Sabrina Richards | Nov 1, 2012 | 8 min read
Enhancing data collection from emulsion PCR reactions: three case studies

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