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Molecule of DNA forming inside the test tube equipment.3d rendering,conceptual image.
EvaGreen® Dye: The Swiss Army Knife of qPCR
Biotium | Mar 1, 2024 | 7 min read
A green fluorescent dye with a novel DNA binding mechanism improves signal-to-noise in different DNA amplification assays.
Combating COVID-19 with Cell-Free Expression
The Scientist | Aug 19, 2020 | 6 min read
Scientists rely on synthetic biology and cell-free expression systems for developing novel approaches to combat the pandemic.
Green and red fluorescent proteins in a zebrafish outline the animal’s vasculature in red and lymphatic system in green in a fluorescent image. Where the two overlap along the bottom of the animal is yellow.
Serendipity, Happenstance, and Luck: The Making of a Molecular Tool
Shelby Bradford, PhD | Dec 4, 2023 | 10+ min read
The common fluorescent marker GFP traveled a long road to take its popular place in molecular biology today.
Creative Emulsification
Sabrina Richards | Nov 1, 2012 | 8 min read
Enhancing data collection from emulsion PCR reactions: three case studies
Fluorescence microscopy image of cells expressing fluorescent biosensors. Green and magenta fluorescence is observed outside of the cell nuclei.
Choosing Fluorescent Reagents for Every Live Cell Application
The Scientist and MilliporeSigma | Nov 30, 2022 | 4 min read
Scientists gain unique insights into active biological processes with specific fluorescent probes, dyes, and biosensors.
PCR Based Cloning Kits: Something For Everybody
Laura Defrancesco | Apr 12, 1998 | 10+ min read
Date: April 13, 1998PCR Based Cloning Kits Table The End Table (PDF Format) PCR has found applications in almost every imaginable facet of molecular biology, and for many applications, looking at a band on a gel is not enough. Sequencing, expressing, mutating--all require cloning. And as it happens, cloning strategies that work for other types of DNA fragments don't work at all well, or require inordinate effort, with PCR fragments. For example, the most commonly used cloning strategy requires
Innovations Expand Lab Power, Uses Of PCR Technique
Ricki Lewis | Jul 25, 1993 | 8 min read
The gene amplification technique invented by genetics researcher Kary Mullis on a moonlit drive through the northern California hills a decade ago--the polymerase chain reaction (PCR)-- continues to revolutionize the life sciences. Uses in molecular biology research and in diagnostic tests are proliferating, and PCR is even bringing a new molecular approach to such fields as paleontology and epidemiology. The following companies are among those supplying PCR-related products for the resear
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Assay for Sickle Cell Anemia Is Repurposed to Diagnose COVID-19
G.B.S.N.P. Varma | Nov 18, 2020 | 5 min read
A CRISPR-based, paper-strip test developed by Indian researchers performs as well as real-time PCR in identifying the presence of SARS-CoV-2 and returns results within an hour.
PCR on the Run
Michael Brush | Jun 25, 2000 | 7 min read
PCR Template Preparation Kits Epicentre Technologies' MasterAmp Buccal Swab DNA Extraction Kit Among the thousands of kits and products available to the life scientist, many provide a means of purifying genomic and plasmid DNA.1 Though most of these prepare highly purified DNA for specific applications such as Southern blotting, fluorescent automated sequencing, and RFLP analysis, some are designed to prepare DNA simply for use as PCR templates. Generally characterized as fast, easy, and eff
Prime Time for Digital PCR
Kelly Rae Chi | Dec 1, 2011 | 8 min read
A rundown of tools on the market and in development

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