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tag emulsion pcr salary reagents cell culture

Fluorescence microscopy image of cells expressing fluorescent biosensors. Green and magenta fluorescence is observed outside of the cell nuclei.
Choosing Fluorescent Reagents for Every Live Cell Application
The Scientist and MilliporeSigma | Nov 30, 2022 | 4 min read
Scientists gain unique insights into active biological processes with specific fluorescent probes, dyes, and biosensors.
Creative Emulsification
Sabrina Richards | Nov 1, 2012 | 8 min read
Enhancing data collection from emulsion PCR reactions: three case studies
Keeping Tabs on Cultured Cells
Lissa Harris(lharris@the-scientist.com) | Mar 13, 2005 | 6 min read
THE CULTURE WITHIN:Photo courtesy of Drs. C.C. Uphoff and H.G. Drexler, DSMZ-German Collection of Microorganism and Cell Cultures, Braunschweig, GermanyHeLa cell line infected with Mycoplasma hyorhinis. Scanning electron micrograph of critical point-dried cell culture grown on cover slips. Note the loop- and rod-like mycoplasmas attached to the host cell membrane. Smaller web-like structures on the cell surface represent microvilli of the cell. Original magnification 3,000×.Mycoplasmas have
Out, Damned Mycoplasma!
Kelly Rae Chi | Dec 1, 2013 | 8 min read
Pointers for keeping your cell cultures free of mycoplasma contamination
PCR Based Cloning Kits: Something For Everybody
Laura Defrancesco | Apr 12, 1998 | 10+ min read
Date: April 13, 1998PCR Based Cloning Kits Table The End Table (PDF Format) PCR has found applications in almost every imaginable facet of molecular biology, and for many applications, looking at a band on a gel is not enough. Sequencing, expressing, mutating--all require cloning. And as it happens, cloning strategies that work for other types of DNA fragments don't work at all well, or require inordinate effort, with PCR fragments. For example, the most commonly used cloning strategy requires
PCR Primed To Spur Chain Of Applications
Holly Ahern | Jun 25, 1995 | 10+ min read
What would you do if your research interests revolved around obtaining DNA from a bacterium preserved for millions of years in the gut of a bee stuck in amber, matching up a murderer to crime- scene blood half a century old, or cloning genes from a 1,000- year-old mummy? Most scientists would first consider PCR--the polymerase chain reaction--as a technique for approaching problems such as these. With PCR, minute quantities of nucleic acids can be amplified millions of times into sufficient qua
Distinguishing Th1 and Th2 Cells
Jeffrey Perkel | May 13, 2001 | 10+ min read
Reagents That Distinguish Th1 and Th2 cells Courtesy of R&D SystemsSchematic representation of cytokines influencing the development of antigen-activated naive CD4+ T cells into Th1 and Th2 cells. Editor's note: Although individual techniques are associated with specific researchers in this article, it should be noted that these investigators commonly use several different techniques to analyze T lymphocyte populations. The human body is constantly under siege. It must defend itself fr
High Fidelity PCR: Enhancing the Accuracy of DNA Amplification
Shane Beck | Jan 4, 1998 | 10 min read
Date: January 5, 1998 Chart 1, Chart 2 n the beginning there was Taq. Actually, there were others before Taq. There were precursory polymerases, such as that from E. coli, that lost their enzymatic activities at elevated temperatures. This shortfall made thermal cycling a time-consuming chore, with the necessity of adding new polymerase after each cycle. Then came the thermostable polymerases such as Taq DNA polymerase, which was isolated from the thermophilic, aerobic bacterium Thermus aquat
Top 10 Innovations 2021
2021 Top 10 Innovations
The Scientist | Dec 1, 2021 | 10+ min read
The COVID-19 pandemic is still with us. Biomedical innovation has rallied to address that pressing concern while continuing to tackle broader research challenges.
Flow Cytometry On-a-Chip
Jeffrey M. Perkel | Jun 1, 2015 | 7 min read
Novel microfluidic devices give researchers new ways to count and sort single cells.

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