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tag culture emulsion pcr pcr digital pcr cell culture

Bio-Rad Launches Vericheck ddPCR Replication Competent Lentivirus and Replication Competent AAV Kits for Cell and Gene Therapy Production
Bio-Rad Laboratories | Feb 27, 2024 | 2 min read
Rapid, Sensitive, and Validated Detection of Viral Replication Genes Using Droplet Digital™ PCR During Cell and Gene Therapy Production
Creative Emulsification
Sabrina Richards | Nov 1, 2012 | 8 min read
Enhancing data collection from emulsion PCR reactions: three case studies
Keeping Tabs on Cultured Cells
Lissa Harris(lharris@the-scientist.com) | Mar 13, 2005 | 6 min read
THE CULTURE WITHIN:Photo courtesy of Drs. C.C. Uphoff and H.G. Drexler, DSMZ-German Collection of Microorganism and Cell Cultures, Braunschweig, GermanyHeLa cell line infected with Mycoplasma hyorhinis. Scanning electron micrograph of critical point-dried cell culture grown on cover slips. Note the loop- and rod-like mycoplasmas attached to the host cell membrane. Smaller web-like structures on the cell surface represent microvilli of the cell. Original magnification 3,000×.Mycoplasmas have
PCR Based Cloning Kits: Something For Everybody
Laura Defrancesco | Apr 12, 1998 | 10+ min read
Date: April 13, 1998PCR Based Cloning Kits Table The End Table (PDF Format) PCR has found applications in almost every imaginable facet of molecular biology, and for many applications, looking at a band on a gel is not enough. Sequencing, expressing, mutating--all require cloning. And as it happens, cloning strategies that work for other types of DNA fragments don't work at all well, or require inordinate effort, with PCR fragments. For example, the most commonly used cloning strategy requires
PCR Primed To Spur Chain Of Applications
Holly Ahern | Jun 25, 1995 | 10+ min read
What would you do if your research interests revolved around obtaining DNA from a bacterium preserved for millions of years in the gut of a bee stuck in amber, matching up a murderer to crime- scene blood half a century old, or cloning genes from a 1,000- year-old mummy? Most scientists would first consider PCR--the polymerase chain reaction--as a technique for approaching problems such as these. With PCR, minute quantities of nucleic acids can be amplified millions of times into sufficient qua
High Fidelity PCR: Enhancing the Accuracy of DNA Amplification
Shane Beck | Jan 4, 1998 | 10 min read
Date: January 5, 1998 Chart 1, Chart 2 n the beginning there was Taq. Actually, there were others before Taq. There were precursory polymerases, such as that from E. coli, that lost their enzymatic activities at elevated temperatures. This shortfall made thermal cycling a time-consuming chore, with the necessity of adding new polymerase after each cycle. Then came the thermostable polymerases such as Taq DNA polymerase, which was isolated from the thermophilic, aerobic bacterium Thermus aquat
A Test Bed for Budding Technologies
Aileen Constans | Jul 4, 2004 | 6 min read
DELETION BY DESIGN:Courtesy of Guci GiaeverThe deletion cassette module used to delete each yeast gene contains two 74-basepair tags upstream and downstream (UPTAG and DNTAG) of the KanMX gene, which confers resistance to the drug geneticin. UPTAG and DNTAG contain 18 basepairs of genomic sequence to flank the yeast's open reading frame, and U1 and U2, or D1 and D2 PCR primers for amplifying a unique 20-basepair TAG region-the so-called molecular barcode. A second round of PCR adds 45 base-pairs
Out, Damned Mycoplasma!
Kelly Rae Chi | Dec 1, 2013 | 8 min read
Pointers for keeping your cell cultures free of mycoplasma contamination
Ready-to-Use Tagged cDNA Clones
AMSbio | Jun 1, 2015 | 1 min read
Available from AMSBIO TrueORF®cDNA clones are tagged cDNA clones for protein studies.
Fluorescence microscopy image of cells expressing fluorescent biosensors. Green and magenta fluorescence is observed outside of the cell nuclei.
Choosing Fluorescent Reagents for Every Live Cell Application
The Scientist and MilliporeSigma | Nov 30, 2022 | 4 min read
Scientists gain unique insights into active biological processes with specific fluorescent probes, dyes, and biosensors.

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