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Getting The Message With RT-PCR
Deborah Wilkinson | Aug 16, 1998 | 10+ min read
Date: July 20, 1998RT-PCR Kits Reverse transcription followed by the polymerase chain reaction (RT-PCR) has become one of the great "workhorse" techniques of today's labs. It is often used as a method for generating needed reagents, including complementary DNA (cDNA) inserts for cloning, cDNA libraries, and templates for in vitro transcription. None of the other commonly used methods for measuring the steady-state levels of individual RNAs (such as Northern or dot blotting, RNase or S1 nuclease
Let's Make a Deal
Stephanie Eberle | Feb 1, 2013 | 8 min read
Six myths about job and salary negotiations and how they may hinder your ability to bargain effectively.
Innovations Expand Lab Power, Uses Of PCR Technique
Ricki Lewis | Jul 25, 1993 | 8 min read
The gene amplification technique invented by genetics researcher Kary Mullis on a moonlit drive through the northern California hills a decade ago--the polymerase chain reaction (PCR)-- continues to revolutionize the life sciences. Uses in molecular biology research and in diagnostic tests are proliferating, and PCR is even bringing a new molecular approach to such fields as paleontology and epidemiology. The following companies are among those supplying PCR-related products for the resear
PCR Primed To Spur Chain Of Applications
Holly Ahern | Jun 25, 1995 | 10+ min read
What would you do if your research interests revolved around obtaining DNA from a bacterium preserved for millions of years in the gut of a bee stuck in amber, matching up a murderer to crime- scene blood half a century old, or cloning genes from a 1,000- year-old mummy? Most scientists would first consider PCR--the polymerase chain reaction--as a technique for approaching problems such as these. With PCR, minute quantities of nucleic acids can be amplified millions of times into sufficient qua
2006 Life Science Industry Awards
Jeffrey M. Perkel and Ishani Ganguli | Apr 1, 2006 | 10+ min read
FEATURELSIA 2006 Our fourth annual event celebrates excellence in life science product development and services BY JEFFREY M. PERKEL AND ISHANI GANGULI It has been said that scientists stand on the shoulders of giants. Generally that refers to researchers' intellectual forebears, but it also is true of the life science industry - technology giants that provide the scientific community with the tools, equipment and
Indiana consortium targeting patent infringers
Ted Agres(tedagres@lycos.com) | Sep 2, 2003 | 4 min read
Group will pursue universities and companies for His-tag payments
High Fidelity PCR: Enhancing the Accuracy of DNA Amplification
Shane Beck | Jan 4, 1998 | 10 min read
Date: January 5, 1998 Chart 1, Chart 2 n the beginning there was Taq. Actually, there were others before Taq. There were precursory polymerases, such as that from E. coli, that lost their enzymatic activities at elevated temperatures. This shortfall made thermal cycling a time-consuming chore, with the necessity of adding new polymerase after each cycle. Then came the thermostable polymerases such as Taq DNA polymerase, which was isolated from the thermophilic, aerobic bacterium Thermus aquat
An illustration of flowers in the shape of the female reproductive tract
Uterus Transplants Hit the Clinic
Jef Akst | Aug 1, 2021 | 10+ min read
With human research trials resulting in dozens of successful deliveries in the US and abroad, doctors move toward offering the surgery clinically, while working to learn all they can about uterine and transplant biology from the still-rare procedure.
map of ukraine
Science Comes to a Halt in Ukraine, at Risk in Russia
Anna Azvolinsky | Mar 4, 2022 | 6 min read
The Russian invasion into Ukraine that began a week ago has forced Ukrainian scientists to abandon their labs and is putting research in Russia in peril.
Think Big, Dress Casual
Karen Hopkin | Nov 7, 2004 | 6 min read
Mike SnyderCourtesy of Michael Marsland"We couldn't get that project funded for the life of us," says Yale University's Mike Snyder of the experiment that, in his opinion, launched the functional genomics era. It was the late 1980s, years before the dawn of DNA microarrays, and Snyder and his colleagues were proposing to use epitope-bearing transposons to tag every protein in yeast. With this collection, the scientists planned to track the positions of all 6,000 yeast proteins, information that

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