ADVERTISEMENT

404

Not Found

Is this what you were looking for?

tag best places to work survey digital pcr dna sequencing reagents

Molecule of DNA forming inside the test tube equipment.3d rendering,conceptual image.
EvaGreen® Dye: The Swiss Army Knife of qPCR
Biotium | Mar 1, 2024 | 7 min read
A green fluorescent dye with a novel DNA binding mechanism improves signal-to-noise in different DNA amplification assays.
Eytan Stibbe wears a headset and a blue shirt while surrounded by computers and other equipment on the International Space Station.
Whenever, Wherever: Taking DNA Amplification Outside the Lab
Hannah Thomasy, PhD | Dec 4, 2023 | 10 min read
Recombinase polymerase amplification lets researchers rapidly replicate DNA in the clinic, in the field, or even in the International Space Station.
Kits to Dye For: A Profile of Sequencing Kits for Automated DNA Sequencers
Michael Brush | Nov 9, 1997 | 9 min read
Date: November 10, 1997 Chart 1 In the long series of events inherent in automated DNA sequencing, cranking out DNA labeled with fluorescent tags is, of course, the most important element of a successful procedure. Without properly labeled sequence ladders to analyze, those expensive, automated DNA sequencers have little to do. So to keep them busy, LabConsumer checked out fluorescent automated DNA sequencing kits from eight manufacturers. The kits profiled exploit two methods for labeling se
Building The Perfect Beast
Debra Swanson | Feb 14, 1999 | 10 min read
Date: February 15, 1999Enhanced PCR Kits and Reagents Sigma's RedTaq DNA Polymerase facilitates gel loading. During the past decade, the polymerase chain reaction (PCR) has become one of the most versatile tools used in the molecular biology laboratory. Applications range from the analysis of genomic DNA samples and the production of DNA fragments for cloning systems to direct DNA sequencing. The reaction requires a minimum of reagents and equipment compared to many lab protocols and can be se
Cycle Sequencing Kits
Michael Brush | Jul 20, 1997 | 10+ min read
When the next time around is always better. Date: July 21, 1997 Comparison Chart DNA sequencing methods have come a long way in 20 years. From the chemical method of Maxam and Gilbert and the dideoxy method of Sanger, DNA sequencing techniques evolved into the "labeling/termination" method that used a modified T7 DNA polymerase such as Sequenase. Propelled by the power and potential of DNA amplification using thermal cycling strategies, earlier DNA sequencing methodologies have increasingly b
PCR Based Cloning Kits: Something For Everybody
Laura Defrancesco | Apr 12, 1998 | 10+ min read
Date: April 13, 1998PCR Based Cloning Kits Table The End Table (PDF Format) PCR has found applications in almost every imaginable facet of molecular biology, and for many applications, looking at a band on a gel is not enough. Sequencing, expressing, mutating--all require cloning. And as it happens, cloning strategies that work for other types of DNA fragments don't work at all well, or require inordinate effort, with PCR fragments. For example, the most commonly used cloning strategy requires
2022 Top 10 Innovations 
2022 Top 10 Innovations
The Scientist | Dec 12, 2022 | 10+ min read
This year’s crop of winning products features many with a clinical focus and others that represent significant advances in sequencing, single-cell analysis, and more.
Getting The Message With RT-PCR
Deborah Wilkinson | Aug 16, 1998 | 10+ min read
Date: July 20, 1998RT-PCR Kits Reverse transcription followed by the polymerase chain reaction (RT-PCR) has become one of the great "workhorse" techniques of today's labs. It is often used as a method for generating needed reagents, including complementary DNA (cDNA) inserts for cloning, cDNA libraries, and templates for in vitro transcription. None of the other commonly used methods for measuring the steady-state levels of individual RNAs (such as Northern or dot blotting, RNase or S1 nuclease
Digital Upgrade
Amber Dance | May 1, 2010 | 7 min read
table thead tr td { border-bottom: 2px solid #000000; }table tr td { font-size: 12px; font-family:"Trebuchet MS", Arial, Helvetica, sans-serif; border-bottom: 2px solid #949494; }#featureArticleContent ul li { font-size: 14px; font-family: "Trebuchet MS", Arial, Helvetica, sans-serif; } By Amber Dance Digital Upgrade How to choose your lab’s next electronic lab notebook The bound, graph-lined laboratory not
DNA Sequencing Industry Sets its Sights on the Future
Edward Winnick | Sep 26, 2004 | 10 min read
THE SANGER METHOD:Single-stranded DNA is mixed with a primer and split into four aliquots, each containing DNA polymerase, four deoxyribonucleotide triphos-phates and a replication terminator. Each reaction proceeds until a replication-terminating nucleotide is added. The mixtures are loaded into separate lanes of a gel and electrophoresis is used to for an illustration of a high-speed DNA sequencer.)What's happening in the DNA sequencing industry? After all, the human genome sequence is done an

Run a Search

ADVERTISEMENT