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tag fluorescent labeling cell molecular biology methods

DNA molecule.
Finding DNA Tags in AAV Stacks
Mariella Bodemeier Loayza Careaga, PhD | Mar 7, 2024 | 8 min read
Ten years ago, scientists put DNA barcodes in AAV vectors, creating an approach that simplified, expedited, and streamlined AAV screening. 
The image shows six different panels containing cells. On each panel, the cells are labelled using a different fluorescent dye that highlights features of a specific organelle within the cells.
Cell Painting: Exploring the Richness of Biological Images
Mariella Bodemeier Loayza Careaga, PhD | Sep 8, 2023 | 4 min read
By coloring different organelles simultaneously, cell painting allows scientists to pick up subtle changes in cell function in response to drugs and other perturbations.
Green and red fluorescent proteins in a zebrafish outline the animal’s vasculature in red and lymphatic system in green in a fluorescent image. Where the two overlap along the bottom of the animal is yellow.
Serendipity, Happenstance, and Luck: The Making of a Molecular Tool
Shelby Bradford, PhD | Dec 4, 2023 | 10+ min read
The common fluorescent marker GFP traveled a long road to take its popular place in molecular biology today.
Microfluidics: Biology’s Liquid Revolution
Laura Tran, PhD | Feb 26, 2024 | 8 min read
Microfluidic systems redefined biology by providing platforms that handle small fluid volumes, catalyzing advancements in cellular and molecular studies.
Microscopic image of a live amoeba.
Illuminating Specimens Through Live Cell Imaging
Charlene Lancaster, PhD | Mar 14, 2024 | 8 min read
Live cell imaging is a powerful microscopy technique employed by scientists to monitor molecular processes and cellular behavior in real time.
Tissue-Clearing Technique Works on Bone
Kerry Grens | Apr 26, 2017 | 1 min read
CLARITY made mouse bones transparent while preserving fluorescent labels so researchers could visualize tagged osteoprogenitors.
Microscopic Bowls Uncover the Secrets of Protein Secretions
Rebecca Roberts, PhD | Jan 30, 2024 | 4 min read
Researchers developed a “test tube” so tiny that it can hold a single cell. These vials enabled them to connect protein secretion levels with surface markers and transcriptome data from the same cell. 
Fluorescence microscopy image of cells expressing fluorescent biosensors. Green and magenta fluorescence is observed outside of the cell nuclei.
Choosing Fluorescent Reagents for Every Live Cell Application
The Scientist and MilliporeSigma | Nov 30, 2022 | 4 min read
Scientists gain unique insights into active biological processes with specific fluorescent probes, dyes, and biosensors.
Fluorescent Labeling Offers Flexibility Without Radioactivity
James Kling | Apr 13, 1997 | 9 min read
There is a war going on to win over the hearts and minds of molecular biologists: Radioactive isotopes-long the gold standard for tagging and later detecting RNA and DNA strands- are being challenged by a new generation of fluorescent labels that promise greater flexibility with fewer disposal problems. SEQUENCE DETECTION SYSTEM: Perkin-Elmer’s ABI Prism 7700 system uses a probe with a reporter and a quencher dye attached to it. With 14C, 32P, 125I, 3H, or some other radioactive atom bui
Discover how a label-free assay redirects time and resources for improved kinetic readouts.
Boosting Throughput with a Label-Free Kinetic Assay
Malvern Panalytical | Jul 31, 2023 | 1 min read
The revolutionary waveRAPID® technique enables biological breakthroughs and improves high throughput drug discovery.

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