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tag long fragment reads digital pcr data standards

Digital PCR as a Universal Molecular Diagnostic Standard
Bio-Rad | Sep 30, 2020 | 6 min read
A reliable primary reference measurement procedure is the key to many forms of measurement across a diverse range of scientific research. The ability of digital PCR to quantify nucleic acids without the need for calibration will likely make it an essential tool for laboratories around the world to calibrate their measurements against. 
Optimizing PCR: Proven Tips and Troubleshooting Tricks
Optimizing PCR: Proven Tips and Troubleshooting Tricks
Tanuka Biswas, PhD | Feb 23, 2024 | 10 min read
Scientists optimize PCR to ensure successful amplification and analysis.
Molecule of DNA forming inside the test tube equipment.3d rendering,conceptual image.
EvaGreen® Dye: The Swiss Army Knife of qPCR
Biotium | Mar 1, 2024 | 7 min read
A green fluorescent dye with a novel DNA binding mechanism improves signal-to-noise in different DNA amplification assays.
Short-Fragment Savvy. NucleoScan 2000 DNA Fragment Analyzer brings flexibility to real-time digital gel imaging
Deborah Noble | Apr 25, 1999 | 3 min read
Nucleoscan 2000 DNA Fragment Analyzer In the past few years, gel electrophoresis of DNA fragments has become an important complement to hybridization and amplification sequencing tools. As variations on PCR, RFLP, mutation sequencing, and sequence and structure analysis emerge, the need for flexible gel electrophoresis systems increases. Until recently, most real-time gel imaging systems have been optimized either for ultrahigh sensitivity and noise suppression or for large-fragment sequencing
PCR: Past, Present, & Future
Jeffrey M. Perkel | Dec 1, 2013 | 6 min read
Highlights from a webinar held by The Scientist to celebrate 30 years of PCR: the technique's invention, quantitative real-time PCR, and digital PCR
Well Read
Jorge Cortese | Oct 1, 2000 | 10+ min read
Microplate Readers Microplate Readers (continued) Laboratory nights are interrupted every so often by the noise of a microplate reader finishing up one plate, then another, and another. But everybody--researchers and students alike--is sound asleep at home, thanks to the newest bunch of microplate reading and juggling machines. The microplate reader was created from the tube spectrophotometer designs of the 1970s to save precious antibody samples. At first clumsy and inaccurate, absorbance mic
Genotyping with PCR
M. Tevfik Dorak | Jun 1, 2007 | 5 min read
How to choose the right approach
Creative Emulsification
Sabrina Richards | Nov 1, 2012 | 8 min read
Enhancing data collection from emulsion PCR reactions: three case studies
A Test Bed for Budding Technologies
Aileen Constans | Jul 4, 2004 | 6 min read
DELETION BY DESIGN:Courtesy of Guci GiaeverThe deletion cassette module used to delete each yeast gene contains two 74-basepair tags upstream and downstream (UPTAG and DNTAG) of the KanMX gene, which confers resistance to the drug geneticin. UPTAG and DNTAG contain 18 basepairs of genomic sequence to flank the yeast's open reading frame, and U1 and U2, or D1 and D2 PCR primers for amplifying a unique 20-basepair TAG region-the so-called molecular barcode. A second round of PCR adds 45 base-pairs
A PCR Primer
Aileen Constans | Jun 10, 2001 | 10 min read
PCR Enhancement Products Courtesy of RedasoftRedasoft's Visual Cloning 2000 includes primer design tools. Courtesy of Sigma-AldrichDirect loading of PCR products onto an agarose gel using Sigma-Aldrich's REDTaq. The art of PCR isn't hard to master. An abundance of products, ranging from relatively low-cost reagents to sophisticated optimization software, exists to meet most, if not all, PCR challenges. This profile looks at commonly used additives, PCR optimization kits and protocols, softwar

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