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tag biochemistry microscopy microplate reader

Hit Parade
Amber Dance | Dec 1, 2012 | 9 min read
Cell-based assays are popular for high-throughput screens, where they strike a balance between ease of use and similarity to the human body that researchers aim to treat.
Luminescence Developments Help Scientists See The Light
James Kling | May 11, 1997 | 9 min read
Biologists are constantly seeking more sensitive assays to detect the presence of organisms or telltale DNA, RNA, and proteins. Although radioactive tags incorporated into the target itself (or into a complementary strand)-and later detected by Geiger counters or film exposures-have traditionally given good sensitivity, the problems of waste disposal and laboratory monitoring have driven a search for alternative tags that have radioactivity's sensitivity but avoid its hazards. Fluorescent tags-
All's Well that Ends Well: A Profile of Specialty Microwell Plates
Brent Johnson | Sep 26, 1999 | 10+ min read
Date: September 27, 1999Table of Specialty Microplates The story of the microplate is one of those tales of history that either has been forgotten or was never clearly understood. According to Barry Lazar of Dynex Technologies, formerly Dynatech Laboratories, the origin of what is now commonly referred to by Dynex's registered trademark of Microtiter plates began with Gyola Takatsy, a Hungarian-born scientist who was trying to scale down serology tests. His first prototype became available in 1
Escaping the Heat
Deborah Fitzgerald | Nov 12, 2000 | 10+ min read
Nonradioactive Kinase Assay Kits Safety concerns and economic considerations have fueled a growing trend in the biomedical sciences: to shun the use of radioactivity when practical. Nonradioactive options for numerous applications have become widely available, including a number of nonradioactive kinase assay kits. Assays from different manufacturers employ a wide range of strategies. Most of these kits utilize antibodies, but two nonimmunochemical approaches use fluorescently tagged substrates
Fluorescence Spectroscopy Methods Reveal Biomolecules' Dynamics
Howard Goldner | Jun 11, 1995 | 8 min read
Dynamics Author: Howard Goldner During the past few decades, fluorescence spectroscopy has developed into an integral technique in many scientific disciplines. In the life sciences, it is implemented extensively in areas such as biochemistry, biophysics, and cell biology for a variety of applications, ranging from basic assay-related quantitative measurements to DNA sequencing. Locates Molecules:Fluorolog-t2 Spectrometer More recently, advances in instrumentation, laser technology, and fluore
Beyond Film: Laboratory Imagers
Jorge Cortese | Apr 1, 2002 | 9 min read
Years ago, researchers had only one data-imaging option: autoradiography. These scientists tagged samples—whether nucleic acid, protein, cell, or tissue—with radioactive labels, and captured images on film. Safety concerns, convenience, and sensitivity, spurred the development of alternative techniques, and today, researchers can choose from a range of options, including fluorescence, chemifluorescence, and chemiluminescence, in addition to autoradiography. Fluorescence occurs when
Immunoassay Techniques Proven To Be Outstanding In Several Fields
Holly Ahern | Oct 1, 1995 | 10 min read
SIDEBAR: Selected Suppliers of Immunoassays and Assay Systems Immunoassays combine principles of immunology and chemistry into tests that are used by scientists in practically every discipline, including fields as diverse as molecular biology and environmental science. In research applications, immunoassays such as immunoblotting (detecting membrane-bound proteins), immunohistochemistry (cell or tissue staining), and enzyme immunoassays provide a sensitive and specific means of detecting targe
Fluorescence Spectroscopy Shedding Light On Complex Biosystems
Holly Ahern | Jun 9, 1996 | 9 min read
Complex Biosystems Spectroscopic detection of fluorescent molecules, either those found naturally or those colored with fluorescent dyes, has created new research possibilities for scientists who study proteins and DNA. With photonic instruments like spectrophotometers and spectrofluorimeters, life scientists can measure the low-intensity light emitted from fluorescent molecules. "Because the character of the light emitted from a molecule changes in respect to the environment the molecule is i
Array of Assays
Michael Brush | Oct 29, 2000 | 10 min read
Protein Assay Manufacturers and Products From the decades-old biuret reaction to the use of complicated fluorescent molecules, the search for an easy, reliable, and stable method for the quantitation of total protein in solution has gone on for more than 80 years. Although several famous, innovative, and useful techniques were developed over that time, the search continues. Bob Vigna, marketing manager for industry leader Pierce Chemical Co. of Rockford, Ill., confirms this observation. "The u
Assays by the Score
Deborah Fitzgerald | May 27, 2001 | 9 min read
Click to view the PDF file: Bead-based Fluorescent Multiplex Protein Analysis Systems Courtesy of LINCO ResearchLabMAP-based systems use internally dyed fluorescent microspheres to analyze as many as 100 different analytes concurrently. Today's competitive, high-paced research environment has stimulated the development of a host of approaches for rapid, cost-efficient analyses of large numbers of samples. In keeping with this trend, methods for simultaneously analyzing multiple species in a g

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