Deborah Wilkinson | Jan 3, 1999 | 3 min read
Schematic illustration of the LexA-based yeast two-hybrid interaction. Source: OriGene Technologies, Inc. 97/98 Catalog. Reprinted with permission. The DupLEX-ATM system from OriGene Technologies is a LexA-based two-hybrid assay for the detection of protein-protein interactions in vivo (J. Gyuris et al., Cell 75:791-803, 1993). The LexA system is conceptually similar to the prototypical GAL4-based two-hybrid system, both of which are based on the bipartate nature of yeast transcriptional acti