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tag lc ms evolution culture

Different colored cartoon viruses entering holes in a cartoon of a human brain.
A Journey Into the Brain
Danielle Gerhard, PhD | Mar 22, 2024 | 10+ min read
With the help of directed evolution, scientists inch closer to developing viral vectors that can cross the human blood-brain barrier to deliver gene therapy.
A Test Bed for Budding Technologies
Aileen Constans | Jul 4, 2004 | 6 min read
DELETION BY DESIGN:Courtesy of Guci GiaeverThe deletion cassette module used to delete each yeast gene contains two 74-basepair tags upstream and downstream (UPTAG and DNTAG) of the KanMX gene, which confers resistance to the drug geneticin. UPTAG and DNTAG contain 18 basepairs of genomic sequence to flank the yeast's open reading frame, and U1 and U2, or D1 and D2 PCR primers for amplifying a unique 20-basepair TAG region-the so-called molecular barcode. A second round of PCR adds 45 base-pairs
The Death of Faith?
Brendan Maher | Apr 1, 2007 | 7 min read
The Death of Faith? Darwin's theory was part of a larger cultural shift towards naturalistic philosophy. Why is he still the target of so many attacks?By Brendan Maher ARTICLE EXTRASSPRING BOOKSStem Cells on ShelvesAn Awkward SymbiosisHigh in the TreesBloody IsleThe Enchantment of EnhancementBooks about BodiesNew Lab Man
Automation Advances in Proteomics
Aileen Constans | Aug 24, 2003 | 10 min read
Courtesy of the Institute for Systems Biology  MOVING FORWARD: The LCQ Deca XP, an electrospray ionization/ion trap mass spectrometer from Thermo Finnigan The sheer number of new protein-focused mass spectrometry (MS) instruments introduced last year is a testament to the growing importance of the technique in proteomics research. Coupled to this trend is a growing need for automation of upfront sample preparation to feed these analytical machines. From the specialized academic lab to hi
Exploring Alternative Codon Usage in Yeast
Kim Smuga-Otto | Aug 18, 2016 | 3 min read
Newly discovered amino acid reassignment could have implications for certain biotech applications and RNA-based evolutionary theories.
Automated Laboratories
Michael Brush | Feb 14, 1999 | 10+ min read
Date: February 15, 1999Table of Robotic Liquid Handler Manufacturers and Table of Pipetting Robots Cytotoxicity studies, ELISA assays, apoptosis assays, peptide library screening--these and many other assays are now performed without human intervention by automated liquid handling systems. Continuing evolution of these machines has produced some very capable and powerful robots, increasing assay throughput to dramatic levels. In this profile, LabConsumer examines the automated liquid handler
Articles Alert
Simon Silver | Jul 8, 1990 | 7 min read
The Scientist has asked a group of experts to comment periodically upon recent articles that they have found noteworthy. Their selections, presented herein every issue, are neither endorsements of content nor the result of systematic searching. Rather, the list represents personal choices of articles the columnists believe the scientific community as a whole may also find interesting. Reprints of any articles cited here may be ordered through The Genuine Article, 3501 Market St., Philadelphia,
Intelligent Redesign
Ishani Ganguli | Aug 1, 2006 | 7 min read
FEATURELab Design   Main image: © Getty ImagesSmaller images: Justin MacNochie Photography/SmithgroupWith billions being spent on new labs and renovations, do shinier labs really make for better science? BY ISHANI GANGULIARTICLE EXTRASRelated Articles:Lab Rehab Redesign and the Bottom Line It's Easy Going GreenAnatomy of an energy-sustainable lab renovationWeb Extra:Lab Rehab: Laying out other options
How to Create a Successful Fish Tale?
A. J. S. Rayl | Aug 19, 2001 | 10+ min read
More than 80 percent of the planet's living organisms exist only in aquatic ecosystems. Some may harbor secrets to human origins, and clues, treatments--perhaps even cures--for human disease. Some are critical bioindicators that portend the health of the biosphere. Yet, overall, scientists know little about the biochemical processes of these life forms. The vast, rich knowledge within the oceans and freshwater systems on Earth remains virtually untapped, because in the world of biological resear
Soybeans Hit Main Street
Barry Palevitz | Mar 19, 2000 | 8 min read
Once a favorite of Chinese emperors, tofu is now big time. From supermarkets to health food boutiques, diet-conscious Americans are buying soybeans, not just as tofu but in infant formula, soy milk, and soy burgers. Soy even has the Food and Drug Administration's seal of approval. Last October the FDA responded to a petition by Protein Technologies International, a St. Louis-based DuPont company specializing in soy products, by authorizing claims that soy protein is good for the heart. Acc

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