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tag stick insects culture genetics genomics

Making Things Grow: Insect Cells, Stem Cells, and Primary Cell Lines All Pose Challenges for Cell Culturists
Laura Defrancesco | Jun 21, 1998 | 5 min read
Date: June 22, 1998 Insect Cell Culture Media, Suppliers of Primary Cell Culture Media Advantages for Protein Expression Studies Since the mid-1950s cultures of insects--cockroaches, fruit flies, and leafhoppers, to name a few--have been the object of quiet study by physiologists and cell biologists. But along came genetic engineering and suddenly insect cultures have been put in the spotlight since they provide advantages over both bacterial and mammalian systems for recombinant protein prod
How Bacteria Interfere with Insect Reproduction
Ruth Williams | Feb 28, 2017 | 3 min read
Scientists identify the genes responsible for bacteria-controlled sterility in arthropods.
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Horizontal Gene Transfer Happens More Often Than Anyone Thought
Christie Wilcox, PhD | Jul 5, 2022 | 10+ min read
DNA passed to and from all kinds of organisms, even across kingdoms, has helped shape the tree of life, to a large and undisputed degree in microbes and also unexpectedly in multicellular fungi, plants, and animals.
Cookbook For Eukaryotic Protein Expression: Yeast, Insect, and Plant Expression Systems
Christopher Smith | Nov 8, 1998 | 10+ min read
Date: November 9, 1998Baculovirus Expression Vectors In the recent past, efforts to elucidate the relationship between protein structure and biological function have intensified. Of particular interest is an understanding of the elements of sequence and structure that mediate specific functions. Often the protein of interest is in low abundance in its natural source and can be difficult to purify and/or unstable--subject to proteolytic cleavage or unfolding/non specific refolding during exhaust
Epigenetics: Genome, Meet Your Environment
Leslie Pray | Jul 4, 2004 | 10+ min read
©Mehau Kulyk/Photo Researchers, IncToward the end of World War II, a German-imposed food embargo in western Holland – a densely populated area already suffering from scarce food supplies, ruined agricultural lands, and the onset of an unusually harsh winter – led to the death by starvation of some 30,000 people. Detailed birth records collected during that so-called Dutch Hunger Winter have provided scientists with useful data for analyzing the long-term health effects of prenat
A Test Bed for Budding Technologies
Aileen Constans | Jul 4, 2004 | 6 min read
DELETION BY DESIGN:Courtesy of Guci GiaeverThe deletion cassette module used to delete each yeast gene contains two 74-basepair tags upstream and downstream (UPTAG and DNTAG) of the KanMX gene, which confers resistance to the drug geneticin. UPTAG and DNTAG contain 18 basepairs of genomic sequence to flank the yeast's open reading frame, and U1 and U2, or D1 and D2 PCR primers for amplifying a unique 20-basepair TAG region-the so-called molecular barcode. A second round of PCR adds 45 base-pairs
Chloroplast Studies Point to Crop Enhancements
Barry Palevitz | Apr 11, 1999 | 5 min read
With news about Dolly and embryonic stem cells the stuff of cocktail party conversation, cloning a transgenic sheep or cow seems like child's play. The recipe is simple: Insert a pet gene into the nucleus of a cultured cell, fuse it with an enucleated egg, and voilà--a cow with high-octane milk. But incorporating genes into nuclear chromosomes isn't the only road to fame and fortune. Animals and plants have other sources of genetic information--their respiratory mitochondria and photosy
Getting to Megabase
Jorge Cortese | Dec 5, 1999 | 7 min read
Large Fragment Cloning Products and Services Gone are the days when all you had to do to get out of grad school was identify and clone a new gene. Besides, with the information rapidly gathered through the Human Genome Project, there will soon no longer be a "new gene." Enormous projects such as sequencing entire genomes have created a need to play with bigger pieces of the puzzle, and a new universe of technologies adapted to large DNA fragments has appeared. Assignment of a new gene to a par
2019 Top 10 Innovations
The Scientist | Dec 1, 2019 | 10+ min read
From a mass photometer to improved breath biopsy probes, these new products are poised for scientific success.
Blotting Technology's Permanence Is Assured As Its Applications In The Laboratory Flourish
Holly Ahern | Nov 27, 1994 | 10 min read
Advanced Biotechnologies Inc. Columbia, MD Advanced Genetic Technologies Corp San Diego, CA Ahlstrom Filtration Inc. Mount Holly Springs, PA American Bioanalytical Inc. Natick, MA Boehringer Mannheim Corp. Indianapolis, IN P.J. Cobert Associates St. Louis, MO Crescent Chemical Inc. Hauppauge, NY DuPont NEN Boston, MA Fluka Chemical Corp. Ronkonkoma, NY Gallard-Schlesinger Industries Inc. Carle Place, NY Hoefer Scientific

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