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tag supercoils genetics genomics

Fishing In A Molecular Sea
Alison Paladichuk | Jan 17, 1999 | 10+ min read
Date: January 18, 1999Labeling Kit Companies Labeling Kit Companies: Details In situ spatial localization of mRNA expressed only after ethylene treatment (left) compared to air grown seedlings used as a negative control (right). The RNA probe to the EIA0305 gene was labeled with fluorescein (NEL633) and visualized with NBT (Image courtesy of New England Nuclear) Remember the days when fishing for a sequence in the E. coli genome seemed like an overwhelming task? Somewhere along the way, it see
Cell-free Transcription and Translation
Gregory Smutzer | Jan 7, 2001 | 8 min read
In Vitro Translation Systems Researchers synthesize recombinant proteins in cell-free extracts to verify the identity of cloned genes, to study protein-protein, protein-nucleic acid, and protein-drug interactions, and to carry out mutagenesis studies. In vitro protein translation studies rely on the efficient and selective transcription of cloned genes in vitro, which is now not only possible, but also routine, thanks to the identification of bacteriophage (phage) DNA-dependent RNA polymerases a

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