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tag quantitative pcr culture cell molecular biology microbiology

Microscopic image of a live amoeba.
Illuminating Specimens Through Live Cell Imaging
Charlene Lancaster, PhD | Mar 14, 2024 | 8 min read
Live cell imaging is a powerful microscopy technique employed by scientists to monitor molecular processes and cellular behavior in real time.
Molecule of DNA forming inside the test tube equipment.3d rendering,conceptual image.
EvaGreen® Dye: The Swiss Army Knife of qPCR
Biotium | Mar 1, 2024 | 7 min read
A green fluorescent dye with a novel DNA binding mechanism improves signal-to-noise in different DNA amplification assays.
Quantitative PCR Update
Wendy Gloffke | Apr 20, 2003 | 9 min read
Courtesy of Applied Biosystems  THE TAQMAN PROCEDURE: Applied Biosystems' TaqMan procedure relies on the 5'-to-3' exonuclease activity of Taq polymerase. The TaqMan probe bears a fluoro-phore at the 5' end and a quencher on the 3' end, rendering the molecule non-fluorescent. During amplification, the probe binds to the template between the two PCR primers. When the polymerase encounters the probe, it starts chewing away at the end, releasing the fluorophore into solution, where it is free
bacteria and DNA molecules on a purple background.
Engineering the Microbiome: CRISPR Leads the Way
Mariella Bodemeier Loayza Careaga, PhD | Mar 15, 2024 | 10+ min read
Scientists have genetically modified isolated microbes for decades. Now, using CRISPR, they intend to target entire microbiomes.
Combating COVID-19 with Cell-Free Expression
The Scientist | Aug 19, 2020 | 6 min read
Scientists rely on synthetic biology and cell-free expression systems for developing novel approaches to combat the pandemic.
Conceptual image of coronavirus, SARS?Cov?2 infects a human cell
Viruses Target Super-Short Protein Motifs to Disrupt Host Biology
Conchita Fraguas Bringas and Jakob Nilsson | May 16, 2022 | 10+ min read
Only recently appreciated as critical components of cellular functions, unstructured stretches of amino acids called SLiMs are key to viral-host interactions.
Close up image of an open notebook with blank pages, a computer keyboard, and a pen.
What's Your Story? Competition Guidelines and Writing Tips
The Scientist | Nov 29, 2023 | 7 min read
Enter for a chance to have your story published on The Scientist’s website! 
Out, Damned Mycoplasma!
Kelly Rae Chi | Dec 1, 2013 | 8 min read
Pointers for keeping your cell cultures free of mycoplasma contamination
Molecular Biology Reagent Kits Simplify Lab Procedures
Ricki Lewis | Feb 7, 1993 | 8 min read
Companies discussed in the accompanying article: Bio-Rad Laboratories 2000 Alfred Nobel Dr. Hercules, Calif. 94547 (800) 4 - BIORAD Fax: (800) 879-2289 Products: Call for catalog BIO 101 Inc. 1060 Joshua Way Vista, Calif. 92083 (800) 424-6101 Fax: (619) 598-0116 Products: phage DNA isolation yeast transformation Geneclean DNA purification Mermaid oligomer purification RNaid RNA purification Circleprep plasmid preparation G nome DNA isolation, for genomic DNA BioWhittaker Inc. 8830 Biggs F
Venus flytrap plants grow in the lab, trigger hairs at the ready.
How the Venus Flytrap Captures Its Prey
Rachael Moeller Gorman | Oct 16, 2023 | 4 min read
Scientists used CRISPR-Cas9 for the first time in a carnivorous plant to prove the role of two ion channels in closing the Venus flytrap’s trap.

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