Aileen Constans | May 27, 2001 | 2 min read
Make way for the Age of SAGE. Introduced in 1995 by Kenneth Kinzler and Bert Vogelstein of the Johns Hopkins University in Baltimore, Md., SAGE™, or Serial Analysis of Gene Expression, is a quantitative method of gene expression analysis based on the idea that an mRNA transcript can be identified by just a short (9-14 base pair) subfragment.1 In the technique, mRNA is isolated, copied into cDNA, tagged at the 3' end with biotin, and then cut with a restriction enzyme. The tagged fragmen