The prominent researcher has been put on administrative leave pending an investigation into unspecified allegations.
Editor's choice in physiology
February 1, 2011|
E. D. Buhr et al., “Temperature as a universal resetting cue for mammalian circadian oscillators,” Science, 330:379-85, 2010. Free F1000 Evaluation
The body’s circadian rhythms are regulated by a “master clock” in the brain, whose tempo is set by light-dark cycles, while the peripheral clocks that control liver metabolism or pituitary hormone release can be reset by a number of stimuli, including temperature. Joseph Takahashi and colleagues at UT Southwestern Medical Center showed that the suprachiasmatic nucleus (SCN) itself was unaffected by temperature, but that it most likely used body temperature to synchronize the peripheral clocks.
Takahashi’s group fused the green luciferase reporter to the mouse clock gene Per2, to watch both the SCN and peripheral clocks glow as they cycled. Changing the temperature of lung and pituitary tissues changed the rhythm of Per2 expression, but didn’t affect the SCN clock. “Light’s a great way to reset the SCN,” says F1000 Member Stacey Harmer, who evaluated the paper, but it’s the “daily cycles in body temperature that help the peripheral oscillators keep time with the brain clock.”
Takahashi’s lab also inhibited heat shock factor 1 (HSF1) in lung and pituitary cultures. Without HSF1, the tissues were unable to respond to increased temperature, implicating heat shock proteins as the thermometer that changes gene expression in peripheral clocks.
How the SCN resists temperature changes is Takahashi’s new problem, he says. He aims to determine the cellular and synaptic mechanisms that might allow the SCN to respond to light, but not to temperature.