Flow Cytometry for the Masses

Tagging antibodies with rare earth metals instead of fluorescent molecules turns a veteran technique into a high-throughput powerhouse.

By | December 1, 2011

Flow cytometry is the current gold standard for identifying cell types within a mixed population. It identifies cells by their unique combinations of markers, which are tagged with antibodies bound to fluorophores and read by the cytometer. The tool is used in fields ranging from developmental biology to immunology, and even in the diagnosis of diseases such as leukemia.

While traditional flow cytometry is fast—analyzing thousands of cells per second—the number of markers that can be measured in a single experiment is limited by overlap in the color spectra of the fluorophores, allowing between 6 and 10 different colors to be measured in a single experiment.

High-end flow cytometers can detect up to 15 different colors, and can even sort cells for later experiments (FACS analysis), though it is limited to markers on the cell’s surface. “Once you start asking questions about signaling networks, the number of parameters you desire to measure goes up exponentially,” says Garry Nolan at Stanford University. But Nolan and colleagues, in what Etienne Joly of INSERM says is “an unbelievable step forward,” have now worked out a way to routinely analyze as many as a hundred different markers in a single cell simultaneously—and they don’t have to be on the surface of the cell, either. Nolan’s preliminary experiments have revealed cell populations that have never been seen before. (Science, 332:687-96, 2011. Read what researchers are saying.)

Anal Chem, 81:6813-22, 2009

The CyTOF mass spectrometer measures isotopes using a different mechanism than the magnetic deflector device depicted here. For a description of the instrumentation, see .

STATS TALK
COMPARING CELL SEPARATION METHODSNUMBER OF PARAMETERSEXPERIENCE NEEDEDCELL SORTINGPRICE OF MACHINESCOST OF LABELING
Flow Cytometry6-152­–3 years (high-end FC)FACS separates cell populations that can be saved and cultured$400-450,000$2 per 1 million cells using fewer than 6 parameters (approximate)
Mass Cytometry< 1003­–6 monthsCells are destroyed$600,000$3/per 1 million cell test, per antibody
 

Correction (December 21): The original version of this article incorrectly stated the comparative pricing information for the two instruments. The article has been corrected and The Scientist regrets the error.

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Comments

Avatar of: Olga Ornatsky

Olga Ornatsky

Posts: 1

December 8, 2011

Hi rpg! Your article "Flow Cytometry for Masses" got the technology  totally wrong. Ha-Ha! Magnetic separation...really! But thanks for the advertisement.

Olga Ornatsky, founder of DVS Sciences Inc, manufacturer of the CyTOF instrument.

Avatar of:

Posts: 0

December 8, 2011

Hi rpg! Your article "Flow Cytometry for Masses" got the technology  totally wrong. Ha-Ha! Magnetic separation...really! But thanks for the advertisement.

Olga Ornatsky, founder of DVS Sciences Inc, manufacturer of the CyTOF instrument.

Avatar of:

Posts: 0

December 8, 2011

Hi rpg! Your article "Flow Cytometry for Masses" got the technology  totally wrong. Ha-Ha! Magnetic separation...really! But thanks for the advertisement.

Olga Ornatsky, founder of DVS Sciences Inc, manufacturer of the CyTOF instrument.

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