Stem cells and cancer cells have enough molecular similarities that the former can be used to trigger immunity against the latter.
What researchers are learning as they sequence, map, and decode species’ genomes
November 18, 2013|
WIKIMEDIA, GERHARD ELSNER
Species: Actinidia chinensis
Genome size: 758 million base pairs (estimated)
Kiwifruit (Actinidia chinensis) are packed with vitamins. Now, scientists may be able to understand how they came to be so nutritious. Researchers from the U.S. and China collaborated to sequence the genome of the Hongyang variety. Their work was published in Nature Communications last month (October 18).
Interrogating a female kiwifruit, the scientists discovered that the genome contains 39,040 protein-coding genes and that this species underwent two whole-genome duplications: one estimated to have occurred, between 73 million and 101 million years ago, the other around 27 million years ago. “The duplication contributed to adding additional members of gene families that are involved in regulating important kiwifruit characteristics, such as fruit vitamin C, flavonoid, and carotenoid metabolism,” study coauthor Zhangjun Fei of Cornell University said in a statement.
“The genome sequence will serve as a valuable resource for kiwifruit research and may facilitate the breeding program for improved fruit quality and disease resistance,” Fei added. The scientists are also hopeful that this genome will provide insight into the evolution of plants like blueberry and persimmon, which are of the same order—Ericales—as kiwifruit.
S. Huang et al., “Draft genome of the kiwifruit Actinidia chinensis,” Nature Communications, doi:10.1038/ncomms3640, 2013.
WIKIMEDIA, NINJATACOSHELL Xanthomonas fuscans
Genome size: 5.1 million base pairs
Xanthomonas fuscans subsp. fuscans (Xff) is part of a large group of bacteria that cause diseases in plants of agricultural importance. Xff specifically targets legumes, causing necrotic lesions on the leaves, stems, seeds, and pods. In an analysis published this month (November 6) in BMC Genomics, researchers from France and Brazil describe having sequenced and annotated the complete Xff genome in order to understand the bacterium’s modes of pathogenicity and evolutionary history.
The team compared the genomes of the 4843-R Xff strain, which was isolated from a plant in a French bean field, with those of several other Xanthomonas species. The researchers found that Xff carried genes that help it readily form biofilms and adjust to living on the surfaces of leaves. Surprisingly, they found that Xff does not have a functional flagella.
“Isolation of such variants from a natural epidemic reveals that either flagellar motility is not a key function for in planta fitness or that some complementation occurs within the bacterial population,” the authors wrote. “Mixing of flagellated and non-flagellated cells in population could also be a strategy to avoid detection by plant defense system by reducing the targets.”
A. Darrasse et al., “Genome sequence of Xanthomonas fuscans subsp. fuscansstrain 4834-R reveals that flagellar motility is not a general feature of xanthomonads,” BMC Genomics, doi:10.1186/1471-2164-14-761, 2013.
WIKIMEDIA, ALESSIO MARRUCCISpecies: Lipotes vexillifer
Genome size: 2.8 billion base pairs
The Yangtze River dolphin or baiji (Lipotes vexillifer) was declared functionally extinct after an extensive survey in 2006. A team of scientists from Nanjing Normal University and Chinese genomics institute BGI sequenced the baiji genome and concluded that humans drove these mammals to extinction. The work was published in Nature Communications last month (October 29).
The baiji genome—sequenced using DNA extracted from the abdominal muscle of a male dolphin stored in a freezer since 1985—revealed unsurprising similarity to bottlenose dolphin and other mammalian genomes. The sequence also showed evidence of a genetic bottleneck, which occurred approximately 10,000 years ago, and a low density of single nucleotide polymorphisms—probably due to low population numbers. Genomic aberrations were unlikely to have played a role in the animal’s extinction, the authors wrote, noting that it was probably “caused by extreme human impacts to the Yangtze River in recent decades” instead.
“The high-quality draft genome of baiji will provide a valuable resource for researchers to uncover the genetic mechanisms underlying extinct species, and will make a great contribution to the protection of endangered species,” project leader Fengming Sun from BGI said in a statement.
X. Zhou et al., “Baiji genomes reveal low genetic variability and new insights into secondary aquatic adaptations,” Nature Communications, doi:10.1038/ncomms3708, 2013.
FLICKR, GIUSEPPE VAGO 2Species: Polynucleobacter necessarius
Genome size: 1.6 million base pairs
The species Polynucleobacter necessarius contains multiple strains, some of which are obligate symbionts of a ciliated protist host—Euplotes—and some that are free-living. In order to understand differences between the bacterial strains, researchers from the U.S. and Italy sequenced the complete genome of a symbiotic P. necessarius, which was published this month (November 12) in Proceedings of the National Academy of Sciences.
A comparative analysis of symbiotic and free-living P. necessarius sequences revealed that both organisms have small genomes. The researchers found that symbiotic genome contains around 1,279 protein-coding genes, a 42 percent reduction from the free-living genome. They pinpointed a large number of pseudogenes in symbiotic P. necessarius as a reason for this reduction. Free-living strains have relatively few metabolic pathways, but symbionts have still simpler metabolic machinery. The scientists also found no evidence that symbiotic P. necessarius exchange DNA with Euplotes.
“We argue that this system will provide an exceptionally useful model for investigations on symbiosis, because of its peculiarities and the commonness and ease of handling of the ciliate hosts,” the authors wrote.
V. Boscaro et al., “Polynucleobacter necessarius, a model for genome reduction in both free-living and symbiotic bacteria,” PNAS, doi:10.1073/pnas.1316687110, 2013.
WIKIMEDIA, U.S. ARMY CORPS OF ENGINEERSSpecies: Ictalurus punctatus
Genome size: 1 billion base pairs (estimated)
Channel catfish (Ictalurus punctatus) are farmed throughout the U.S., so understanding their genetic makeup is of agricultural and economic importance. Researchers from Auburn University in Alabama published a whole-genome analysis of I. punctatus compared with four model fish species—zebrafish, medaka, Tetraodon, and stickleback. Their work was published in BMC Genomics this month (November 11).
The team found the highest level of chromosome conservation between catfish and zebrafish—followed by stickleback, medaka, and Tetraodon, respectively—and identified homologous chromosomes between catfish and each of the other species. The researchers also determined syntenic regions in the genome of catfish and the other species, which they wrote would provide “valuable information for whole-genome assembly and annotation in catfish.” The scientists also were able to identify large numbers of genes in catfish homologous to genes in other species—14,035 in zebrafish, 9,949 in medaka, 9,465 in stickleback, and 7,181 in Tetraodon.
The comparative genomics approach “not only allows better understanding genome arrangement during evolution, but also benefits the discovery or confirmation of orthologies among species,” the authors wrote.
Y. Jiang et al., “Whole genome comparative analysis of channel catfish (Ictalurus punctatus) with four model fish species,” BMC Genomics, doi:10.1186/1471-2164-14-780, 2013.