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Scientists create a way to isolate mRNA from a single living cell within a tissue.
April 1, 2014|
© GEORGE RETSECKNeighboring cells with the same morphology in the same tissue—neurons in the brain, for example—may be unique in their mRNA and protein-expression profiles. While cell individuality is increasingly appreciated, current whole-transcriptome techniques cannot analyze a single cell within a tissue without perturbing the cell’s neighbors or having to use fixed tissue samples.
Cleverly combining several molecular tools into a single, multitasking molecule, neurobiologist James Eberwine and chemist Ivan Dmochowski, both at the University of Pennsylvania, created the transcriptome in vivo analysis (TIVA) tag. It’s composed of a light-activated hairpin oligonucleotide that can enter a cell and capture its mRNA profile without contamination from neighboring cells. “The TIVA tag in its closed hairpin structure looks like a pocket knife with tools folded inside. Upon photoactivation, TIVA opens up and the RNA capture strand is revealed,” says Dmochowski.
Before opening the tag’s hairpin loop, fluorescence resonance energy transfer (FRET) between dyes attached to the RNA hairpin confirms the presence of the tag inside the cell. Because many cells in a tissue might take up tags, the trick to analyzing a single cell is to activate just the cell of interest by shining a blue laser light on it alone. This photoactivation causes the hairpin to break apart, exposing a chain of 18 uracil (U) nucleotides that can bind the poly(A) tail of mRNA molecules. The poly(U) chain of the tag retains a biotin molecule that allows researchers to retrieve and analyze the mRNAs after breaking open the cell.
© GEORGE RETSECK “This is a very elegant, although technically challenging, approach to gain insight on the transcriptome of a single cell in its native environment,” said Zhengjian Zhang, molecular biologist at the Howard Hughes Medical Institute Janelia Farm Research Campus who was not involved in the research. (Nat Meth, 11:190-96, 2014)
A pipette suctions the cytoplasm contents from
a single cell.
|Technically easier to perform than TIVA||Potential contamination of RNA from neighboring cells; injury could elicit transcriptional changes||Cells larger then 10 μm in diameter (smaller cells are much more difficult)|
|Transcriptome in vivo analysis (TIVA)||Photoactivated tag retrieves mRNA for whole-transcriptome analysis of a single cell.||Noninvasive, single-cell precision; potential for subcellular analysis||
Technical expertise required
to synthesize tag