Sense and Sensibility

Tailored combinations of a reporter and a ligand-binding domain allow for sensing just about any molecule of interest.

By | May 1, 2016

SENSOR SET-UP: To detect a small molecule of interest (the ligand), a conditionally stable ligand-binding domain (LBD) is fused to a reporter, such as green fluorescent protein (GFP). The complex degrades if the ligand is not present (1), and activates the reporter when it is (2). In another demonstration of this sensor, researchers connected the LBD to a DNA-binding domain (DBD) (3). When the ligand is present, the DBD hooks onto to a site in the genome (red), which results in the expression of a specified reporter gene (yellow) (4).© GEORGE RETSECK; ELIFE, 4:E10606, 2015See the full article here.

 

 

Add a Comment

Avatar of: You

You

Processing...
Processing...

Sign In with your LabX Media Group Passport to leave a comment

Not a member? Register Now!

LabX Media Group Passport Logo

Popular Now

  1. Can Young Stem Cells Make Older People Stronger?
  2. Thousands of Mutations Accumulate in the Human Brain Over a Lifetime
  3. CRISPR to Debut in Clinical Trials
  4. Two Dozen House Republicans Do an About-Face on Tuition Tax