Microbial Ice-Makers

How one bacterium turns water into ice at nonfreezing temperatures

By Jef Akst | April 26, 2016

FLICKR, JAMES MANNPseudomonas syringae uses a special cell-wall protein as a mold for arranging water molecules into ice, even at temperatures above water’s normal freezing point, according to a study published last week (April 22) in Science Advances. Specifically, the protein, called inaZ, has alternating water-repelling and water-attracting regions, which push and pull on water molecules into an ice-like crystal. In the lab, P. syringae was able to crystallize water at 4° C; in nature, the bacteria are able to freeze water at around –2° C, according to Science News. But even that is still several degrees higher than the normal freezing point of pure water (around –40° C), The Verge reported.

“People have tried to understand how [the bacteria] control ice nucleation and they’ve done theoretical and computational studies,” study coauthor Tobias Weidner, a bioengineer at the Max Planck Institute for Polymer Research, told The Verge. “And now, this is the first time we have experimental data that shows this actually happens.”

Understanding this ice-making ability of P. syringae, which live on crops and other plants, could help researchers protect these organisms from frost damage resulting from the formation of ice crystals insides the plant’s tissues. “They nucleate ice to attack plant cells. They’re also used in artificial snowmaking. Then, they’re also involved in climate processes,” Weidner told The Verge. “It’s pretty spectacular.” The information could also inform scientists’ understanding of how P. syringae create clouds and rain, or help those who use the bacteria to make artificial snow at ski resorts.

However, because the study used a powder called Snowmax, which contains dead P. syringae along with nutrients intended to help the microbes grow before they’re freeze-dried and irradiated, more work is needed to confirm how these bacteria function in the field, biologist and ecologist Cindy Morris of the French National Institute for Agricultural Research (INRA) told The Verge. “This paper tells us about the physics of what’s going on,” she said, but “it doesn’t tell us any more than we knew before about the role of these bacteria in precipitation.” 

Clarification (April 26): This story has been updated to cite The Verge for the freezing point of water as –40° C.

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Avatar of: Miguel Vicente

Miguel Vicente

Posts: 3

April 26, 2016


Avatar of: St James

St James

Posts: 4

April 26, 2016

I am confused as Miguel Vicente.  I have alway thought water froze at 0 degrees C.

Avatar of: dumbdumb


Posts: 100

April 27, 2016

You are both mistankely referring to the normal H2O. The H2O in the study is that special one! Don't you remember?

BTW, I worked wth this protein about ten years ago. And I seem to remember that its function was pretty much known back then.

I guess, if you use "interface-specific sum frequency generation spectroscopy" (must be a fancy technique by the sound of it) to study it, you can give it a new spin and get published in a fancy journal!

It could even get reported, in the even fancier, the-scientist! ;-)~

Avatar of: ebitnet


Posts: 1

April 28, 2016

I'm not sure how the "-40?C" came about...I think the author meant 40 deg F.  

The water in this case freezes at +4 deg C due to alternating hydrophobic/hydrophilic domains in the inaZ protein.  

The sum-frequency generation SFG spectroscopy is not nescesarily new or exotic (at this point)...but is perfectly suited for detecting interfacial ordering.

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