Taxonomic differences in bacterioplankton amino acid uptake
July 1, 2016|
EDWARD DELONG, DAVID KARL, NANCY HULBIRT
S. Bryson et al., “Proteomic stable isotope probing reveals taxonomically distinct patterns in amino acid assimilation by coastal marine bacterioplankton,” mSystems, doi:10.1128/mSystems.00027-15, 2016.
Marine systems fix about 50 gigatons of carbon each year, of which about half is processed by heterotrophic microbial communities. But relatively little is known about the role of individual taxa in the assimilation and metabolism of carbon compounds. So Samuel Bryson, a graduate student in Ryan Mueller’s lab at Oregon State University, set out to test a method that could improve taxon-specific measurements of carbon uptake and usage.
Bryson and his colleagues added 13C-labeled amino acids to seawater samples of bacterioplankton collected at two different locations off the western coast of the U.S. The team then used metagenomics and mass spectrometry to analyze various bacterial orders’ incorporation of 13C into peptides to see “who is taking up what,” says Bryson.
The researchers found that some orders, such as Alteromonadales, incorporated 13C-amino acids into biomass more rapidly than more-dominant orders like Rhodobacteriales and Flavobacteriales. “I think it’s using some really cutting-edge technology to get at an important question in microbial oceanography,” says David Kirchman, a marine microbiologist at the University of Delaware, adding that data on how bacteria share the job of carbon assimilation will help scientists better understand carbon cycling in marine systems.
So far, the method has “confirmed and validated other predictions and observations” about taxonomic differences in amino acid assimilation, says Bryson. The next step will be to increase the complexity of the experiments, by “looking at how taxa respond across a variety of substrates,” he says.
July 8, 2016
Re.chronic fatigue,where to get info
A year ago I wasi in ICU for 3 days with sepsis due to ecoli. Only symptom was extreme fatigue. MDs did not know how I got it but it was cleared up in 3 days and I was sent home with no explanation. I had suggested that a colonoscopy which had been performed 2 weeks before may have had something to do with it because of similarity post procedure symptom (fatigue and excessive sleep) and sepsis diagnosis. Still extremely tired and sleepy a year later and I would like to know if this is something that could be helped.
i have no idea who I am sending this message to but my doctors aren't interested ans this is the first thing I have read on the subject.