Scientists Catch Translation in the Act

Newly developed techniques from four different groups rely on the same basic steps to track translation in live cells.

By Ruth Williams | October 1, 2016

TRANSLATION ORIENTATION

Four independent teams of researchers have designed methods to observe translation in living cells, all based on the same basic approach. They engineered an mRNA to fold repeatedly into so-called stem loops in its 3’ untranslated region (blue). A fluorescent protein (pink) binds to these loops to allow for mRNA tracking. To observe the newly formed peptide, the researchers built in repeated peptide sequences (purple) that bind green fluorescent protein (green).

© GEORGE RETSECK

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