Infographic: How to Build a Synthetic Sensor

Scientists designed a genetic sensor-and-readout system, based on detecting a transcription factor, that performs a custom cellular activity.

By Ruth Williams | December 1, 2016

© LUCY READING-IKKANDA

Synthetic Circuit

In this example of a typical transducer, the genetic construct (top) includes an amplifier response element and a carefully positioned transcription factor response element upstream of a promoter that drives expression of a fusion gene (the combined effector of choice and an amplifier). In the presence of a specific endogenous transcription factor, which binds to the transcription factor response element, the fusion gene is expressed. Cleavage of the fusion protein releases the amplifier, which together with the transcription factor drives much stronger expression. The system is like a positive feedback loop, but neither the transcription factor nor the amplifier alone can drive strong expression—they need each other.

Read the full story.

Add a Comment

Avatar of: You

You

Processing...
Processing...

Sign In with your LabX Media Group Passport to leave a comment

Not a member? Register Now!

LabX Media Group Passport Logo

Popular Now

  1. Two University of Rochester Professors Resign in Protest
  2. Dartmouth Professor Investigated for Sexual Misconduct Retires
  3. Theranos Leaders Indicted For Fraud
    The Nutshell Theranos Leaders Indicted For Fraud

    Federal prosecutors filed criminal charges that allege the company’s promise to revolutionize blood testing swindled investors out of hundreds of millions of dollars and put patients in danger.

  4. Koko the Signing Gorilla Dies at 46