A few months after the American Chemical Society won its lawsuit against the pirate site, the game of virtual whack-a-mole continues.
E. coli that make indoles protect older worms, flies, and mice from frailty.
August 28, 2017|
WIKIMEDIA, NIHMice, like any other animal, do not age gracefully—they lose weight, move less, and their coats become patchy. But mice of a particular strain in Daniel Kalman's lab at Emory University School of Medicine ward off these declines. The secret, Kalman has found, is in the animals’ guts.
In a study published in PNAS this week, Kalman and colleagues show that indole, a molecule produced by commensal bacteria, extends “healthspan” not only in mice but in the nematode worm Caenorhabditis elegans and in fruit flies as well. Taking away the bacterial production of the molecule ablated these anti-aging gains.
Healthspan—the time before age-related infirmities strike—is tightly correlated with lifespan. Yet, “aging research for a long time has examined lifespan without examining the effect on health,” says Heidi Tissenbaum of the University of Massachusetts Medical School who was not involved in the work. “This study is pushing the boundaries, especially because it examines the effect of indoles on healthspan across three different species.”
Indoles serve as molecular messengers between bacteria and other species, controlling various aspects of bacterial physiology such as biofilm and spore formation and virulence. “Indoles are likely only one of many classes of molecules used by commensal bacteria and plants to communicate with us,” says Kalman. “Indoles have similar functions in animals from diverse phyla separated by hundreds of millions of years of evolution.”
Over 17 years of experiments with C. elegans, Kalman and his group have observed that indoles secreted by bacteria induce protection against stressors in the worms. “We had been looking for some time at how indoles regulate stress sensitivity, which is part of health span,” writes Kalman in an email to The Scientist. The authors also wanted to test whether indoles affect other aspects of healthspan such as fecundity, motility, and survival.
So Kalman and his team used antibiotics to wipe out naturally occurring gut microbes in C. elegans, and raised the worms in cultures containing a strain of Escherichia coli that can colonize the gut and synthesize indoles at greater amounts than other strains. Other animals were grown on E.coli that could not make indoles.
The indoles from E. coli did not significantly increase the lifespan of the worm, but the aged C. elegans showed “thrashing motility” comparable to that of a younger worm. The indole-supplied nematode could also eat bigger meals than its deprived counterparts because its pharyngeal muscles—the muscles that take in food in nematodes—functioned without a hitch, unlike the weakened muscles in normal aged worms.
The authors also found that indoles triggered the expression of a “distinct set of healthspan genes” in C. elegans. The gene expression profile in nematodes grown with indole-producing E. coli was more similar to a typical young C. elegans, rather than a normal aged worm. Indoles also induced genes associated with egg production in older C. elegans, extending reproductive age.
Applying the same experiments to insects and mammals, the researchers found that Drosophila melanogaster harboring indole-producing E. coli performed better in climbing assays, and survived heat stress of 38 °C better than control flies. Mice with indole-producing E.coli were more resistant to radiation than control mice, and had more youthful activity levels, mobility, grip strength, and coats.
“The study is statistically extremely robust,” says Dario Valenzano of the Max Planck Institute for Biology of Ageing. “The paper convincingly shows that the lack of indoles is detrimental to healthspan. What will be interesting is to see if the same effect holds good with wild-type animals treated [extraneously] with indoles.”
In both C. elegans and D. melanogaster, indole functions by binding to the AHR (aryl hydrocarbon receptor), a transcription factor with a variety of organ-specific functions. “The AHR recognizes a diverse array of molecules, not just indoles,” says Kalman. “[The] evolution of variants is likely selected for by the particular molecules an organism encounters.”
The human body has a variant of the AHR. In humans and other mammals, indoles absorbed from plant dietary sources, or produced by intestinal microbiota through catalysis of dietary tryprophan, are found in small quantities all over the body. In mice, they have been shown to limit the virulence of pathogenic bacteria and protect the intestinal lining.
Molecules such as indoles that affect healthspan and help the body cope with stressors could potentially serve as therapeutics to help treat various maladies. They could also act as indicators of body health, and may find use in predicting and diagnosing gut-related disease, says Kalman.
That said, “it is a long road to go from the data we present to a drug,” he cautions. “That takes careful development, testing, and safety and efficacy trials.”
R. Sonowal et al., “Indoles from commensal bacteria extend healthspan,” PNAS, doi:10.1073/pnas.1706464114, 2017.
August 28, 2017
The food energy-dependent pheromone-controlled physiology of reproduction in species from microbes to humans biophysically constrains the virus-driven degradation of messenger RNA that links mutations to all pathology in all living genera.
There is no such thing as transgenerational epigenetic inheritance of morphological or behavioral phenotypes outside the context of feedback loops that link food odors from the metabolism to pheromones and biophysically constrained viral latency. Feedback loops link odor and pheromone signaling with reproduction