Genetic Variant Detection in Cancer: Using ISH to Track Tumor Evolution

Analyzing intratumor heterogeneity is essential for predicting therapeutic response. 

By The Scientist Creative Services Team | December 14, 2017

Genetic Variant Detection in Cancer: Using ISH to Track Tumor Evolution from thescientistllc on Vimeo.

Intratumor heterogeneity (ITH) is a major underlying cause of therapy resistance and disease recurrence, and is a read-out of how a tumor has grown. Current methods to analyze genetic ITH rely on the sequencing of ‘bulk’ or flow-sorted populations, in which the spatial context of tumor subclones is not preserved, and rare subclones may not be detected. These shortfalls can be addressed with BaseScope™ ISH–a unique mutation-specific RNA in situ hybridization assay. The BaseScope assay represents a significant technical advance for in situ mutation detection, and provides new insight into the mechanisms of tumor evolution with potential ramifications for selecting patients for treatment. Join us to learn more about this new approach to ITH analysis.


Topics to be covered:

  • How ITH influences treatment successes and failures
  • How the BaseScope ISH assay enables reliable detection of ITH

Meet the Speakers:

Ann Marie Baker, PhD
Centre for Tumour Biology, Barts Cancer Institute
Queen Mary University of London



Courtney Anderson, PhD
Group Leader Applications
Advanced Cell Diagnostics



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