Spatially controlled polymerization of the cytoskeleton protein actin generates the motile force required for biological processes such as the formation of cellular protrusions like lamellipodia, cancer cell metastases, and the intracellular movements of bacterial and viral pathogens within the cytosol of infected cells. In the May 5
Giardini et al. developed a membrane-based reconstituted system in which large artificial lipid vesicles coated with the protein ActA from the bacterium Listeria monocytogenes — a facultative intracellular pathogen capable of rapid movement through the host cell cytoplasm — were propelled by actin polymerization in cytoplasmic extracts. The model closely mimics in vivo conditions. To move within the cytosol of infected cells, Listeria ...
