This webinar, brought to you by Bio-Rad, will be hosted live and available on-demand.
Tuesday, October 6th, 2026
11:00 AM - 12:00 PM ET
A well-designed qPCR assay is the foundation of accurate, reproducible results. But designing the right primers, optimizing reaction conditions, and validating assay performance can be both time-consuming and challenging.
In this joint webinar, Sarah Daniel, PhD, Bio-Rad Field Applications Scientist, will share practical strategies for developing robust qPCR assays, guiding attendees through assay design, optimization, and implementation. For the second part, PhD candidate Bryant Colin from the University of Chicago will then present a real-world case study on building a five-target multiplex assay to read T cell responses across a 384-well screen across many samples, covering the design decisions, validation and troubleshooting behind it.
Whether you're developing a single assay or planning a high-throughput gene expression study by scaling either the number of genes analysed or the number of samples using a smaller panel, this webinar will provide practical guidance on approaches to reduce assay development time, improve reproducibility, and generate reliable qPCR data faster.
Topics to be covered
Design assays with confidence
- Best practices for designing primers and probes
- Where to obtain high-quality assay components/In-silico tools available to generate robust assays
- Common design pitfalls and how to avoid them
Optimize assay performance
- How temperature gradients can rapidly identify optimal annealing conditions
- Using melt curve analysis to verify assay specificity
- Practical tips for developing reproducible, publication-quality assays
Accelerate your workflow and sample throughput with prevalidated assays
- When it makes sense to use validated PrimePCR™ assays instead of designing assays from scratch
- How predesigned and customizable PrimePCR panels can simplify experimental setup
- Strategies for rapidly screening many samples within disease-specific pathways, enabling large-scale qPCR studies with minimal assay development time
 | Sarah Daniel, PhD |
 | Bryant Colin |
The content presented in these sessions is intended for research use only and is not intended for use in diagnostic procedures. The views and opinions expressed in the recordings are those of the individual presenters and do not necessarily reflect the official policy, position, or claims of Bio-Rad Laboratories.
Any clinical applications, test validations, or performance claims discussed are the sole responsibility of the presenting institution or speaker and do not reflect the views or endorsements of Bio-Rad Laboratories.
Any reference to clinical use, patient testing, or performance claims reflects the practices and experience of the individual institutions or researchers and should not be interpreted as endorsement by Bio-Rad.
For Research Use Only. Not for use in diagnostic procedures.
