T.A. Rooney, E.J. Sass, A.P. Thomas, "Agonist-induced cytosolic calcium oscillations originate from a specific locus in single hepatocytes," Journal of Biological
Andrew Thomas (Thomas Jefferson University, Philadelphia): "Recent improvements in digital imaging microscopy have resulted in revolutionary advances in our understanding of intracellular calcium homeostasis. These techniques have demonstrated that activation of receptors that act via the second messenger inositol 1,4,5-trisphosphate can result in increases in intracellular calcium ([Ca2+]i), which are encoded in the form of [Ca2+]i oscillations in individual cells. In addition to the temporal organization provided by [Ca2+]i oscillations, we demonstrated a further level of organization of Ca2+ signaling in the spatial domain within individual primary cultured hepatocytes.
"Hormone-induced [Ca2+]i oscillations do not occur synchronously throughout the cell, but they originate as waves of Ca2+ from a specific region adjacent to the cell membrane and then they are self-propagated through the cell.
"Furthermore, the oscillatory...