These papers were selected from multiple disciplines from the Faculty of 1000, a Web-based literature awareness tool
J. Lu et al., "MicroRNA expression profiles classify human cancers,"
This article makes the surprising discovery that microRNA-expression profiles can be better predictors of cancer outcome than mRNA profiles. This conclusion is based on the use of a novel, bead-based flow-cytometry approach to examine the expression of 217 miRNAs from 334 samples.
- Miles Wilkinson
I. Bentwich et al., "Identification of hundreds of conserved and nonconserved human microRNAs,"
Eighty-nine human microRNAs are confirmed by cloning and sequencing. The miRNAs are grouped into families on the basis of hairpin sequence similarities, and are located on the genome in two nonconserved clusters.... The authors bring the total number of human miRNAs to 311, suggesting that the world of human miRNAs is larger than initially believed and a substantial portion of miRNAs are primate-specific.
- Claudia Bagni
M.A. Pufall et al., "Variable control of Ets-1 DNA binding by multiple phosphates in an unstructured region,"
This research describes an example of regulation of protein activity by phosphorylation that yields a graded (rather than all-or-none) effect, and underscores the link between protein structure and function. An unstructured domain of the Ets-1 transcription factor is subject to phosphorylation at multiple sites in response to Ca2+ signaling, with increased phosphorylation conferring greater rigidity to the domain and correspondingly decreasing the DNA binding affinity.
- Helen Chamberlin