These papers were selected from multiple disciplines from the Faculty of 1000, a Web-based literature awareness tool
S. Cabantous et al., "Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein,"
A split [green fluorescent] protein-tagging and detection system was developed that can be easily used in high-throughput screens to obtain soluble, well-folded (recombinant) proteins. Additionally, the system holds great promise for the analysis of protein trafficking/protein localization (e.g., in eukaryotic cells) and for time-resolved monitoring of promoter coexpression.
- Jan Kok
S. Takyar et al., "mRNA helicase activity of the ribosome,"
Using total ribosome reconstitution, stepwise in vitro translation, and primer extension assays, the authors demonstrate that the helicase activity that accomplishes the crucial function of unwinding double-stranded mRNA resides within the downstream tunnel of the ribosome itself. Molecular...