Interdisciplinary research

These papers were selected from multiple disciplines from the Faculty of 1000, a Web-based literature awareness tool http://www.facultyof1000.com.S. Cabantous et al., "Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein," Nat Biotechnol, 23:102–7, Jan. 5, 2005.A split [green fluorescent] protein-tagging and detection system was developed that can be easily used in high-throughput screens to obtain soluble, well-folded (recombinant) proteins.

The Scientist Staff
Mar 27, 2005
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These papers were selected from multiple disciplines from the Faculty of 1000, a Web-based literature awareness tool http://www.facultyof1000.com.

S. Cabantous et al., "Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein," Nat Biotechnol, 23:102–7, Jan. 5, 2005.

A split [green fluorescent] protein-tagging and detection system was developed that can be easily used in high-throughput screens to obtain soluble, well-folded (recombinant) proteins. Additionally, the system holds great promise for the analysis of protein trafficking/protein localization (e.g., in eukaryotic cells) and for time-resolved monitoring of promoter coexpression.

- Jan KokGenetisch Instituut, Netherlands

S. Takyar et al., "mRNA helicase activity of the ribosome," Cell, 120:49–58, Jan. 14, 2005.

Using total ribosome reconstitution, stepwise in vitro translation, and primer extension assays, the authors demonstrate that the helicase activity that accomplishes the crucial function of unwinding double-stranded mRNA resides within the downstream tunnel of the ribosome itself. Molecular...

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