Timing xenotransplantation

© 2005 National Academy of SciencesIsraeli researchers report that embryonic pig tissues used for liver, pancreas, and lung transplants need to come from very specific windows of time in embryonic development.1 The findings offer new insights into organogenesis and might help explain past failures in xenotransplantation, says coauthor Yair Reisner of the Weizmann Institute of Science in Rehovot, Israel.Reisner and his colleagues transplanted pig tissue precursors from embryonic day (E)21 to

Mar 14, 2005
Charles Choi
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© 2005 National Academy of Sciences

Israeli researchers report that embryonic pig tissues used for liver, pancreas, and lung transplants need to come from very specific windows of time in embryonic development.1 The findings offer new insights into organogenesis and might help explain past failures in xenotransplantation, says coauthor Yair Reisner of the Weizmann Institute of Science in Rehovot, Israel.

Reisner and his colleagues transplanted pig tissue precursors from embryonic day (E)21 to E100 into immune-deficient mice to identify the optimal windows for the growth of liver, pancreas, and lung. They saw optimal liver growth and function at E28, with enzyme-linked immunosorbent assay (ELISA) showing rapid decrease of secreted albumin levels from implants past that stage. In contrast, development of mature lung tissue was not observed via stained micrographs until relatively late in gestation at E56. Maximal pancreas growth and function was seen from E42 to E56, with ELISA revealing reduced insulin secretion capacity before and afterward.

"This study has identified critical checkpoints in [organ] development," says Bernhard Hering of the University of Minnesota in Minneapolis, "and it has important implications to help identify what transcription factors get activated here and there, and what factors are needed to move from one stage to the next."