Familiar fluorochromes can make panel design feel more manageable, but the most familiar option is not always the best fit. A fluorochrome that works well for a highly expressed, clearly defined antigen may not provide the brightness or separation needed for a marker with low or variable expression.
With hundreds of fluorochromes available, researchers need a way to compare their performance and assign selected dyes to appropriate markers. This compendium approaches that challenge from two directions. The first resource provides performance rankings and pairing recommendations based on antigen density and expression profile. The second presents performance data for a newly developed fluorochrome family, examining how these dyes may help address challenges related to resolution, spillover, photostability, and compatibility in multicolor panel design.
Download this compendium to explore
- How spillover and resolution affect fluorochrome performance
- Which dye properties matter for low or variable antigen expression
- How antigen density and expression profile guide fluorochrome assignment
- How an advanced fluorochrome family addresses limitations associated with PE and PerCP tandem dyes
